ArticlePlant physiology2001
Physiological and molecular biological characterization of intracellular carbonic anhydrase from the marine diatom Phaeodactylum tricornutum.
Article in Plant physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 88 citations in OpenAlex.
- Diatoms in low pH environments: diversity, adaptations, mechanisms, ecological roles, and applications.Archives of microbiology · 2025Review
- Low-CO2-inducible bestrophins outside the pyrenoid sustain high photosynthetic efficacy in diatoms.Plant physiology · 2024Article
- Adaptive responses of marine diatoms to zinc scarcity and ecological implications.Nature communications · 2022Article
- Phytate as a Phosphorus Nutrient with Impacts on Iron Stress-Related Gene Expression for Phytoplankton: Insights from the DiatomApplied and environmental microbiology · 2022Article
- Structural Contour Map of the Iota Carbonic Anhydrase from the DiatomInternational journal of molecular sciences · 2021Article
- Structural and thermodynamic analysis of factors governing the stability and thermal folding/unfolding of SazCA.PloS one · 2021Article
- Regulation of Carbon Metabolism by Environmental Conditions: A Perspective From Diatoms and Other Chromalveolates.Frontiers in plant science · 2020Review
- Mechanisms of carbon dioxide acquisition and COPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- Evolutionarily distinct strategies for the acquisition of inorganic carbon from seawater in marine diatoms.Journal of experimental botany · 2017Article
- Ocean acidification modulates expression of genes and physiological performance of a marine diatom.PloS one · 2017Article
- Localization of putative carbonic anhydrases in the marine diatom, Thalassiosira pseudonana.Photosynthesis research · 2014Article
- A chloroplast pump model for the CO2 concentrating mechanism in the diatom Phaeodactylum tricornutum.Photosynthesis research · 2014Article
- CO2 acquisition in Chlamydomonas acidophila is influenced mainly by CO2, not phosphorus, availability.Photosynthesis research · 2014Article
- The central carbon and energy metabolism of marine diatoms.Metabolites · 2013Article
- SLC4 family transporters in a marine diatom directly pump bicarbonate from seawater.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Redox regulation of carbonic anhydrases via thioredoxin in chloroplast of the marine diatom Phaeodactylum tricornutum.The Journal of biological chemistry · 2012Article
- CO(2)-cAMP-responsive cis-elements targeted by a transcription factor with CREB/ATF-like basic zipper domain in the marine diatom Phaeodactylum tricornutum.Plant physiology · 2012Article
- Recent progresses on the genetic basis of the regulation of CO2 acquisition systems in response to CO2 concentration.Photosynthesis research · 2011Review
- Photosynthetic characteristics of a multicellular green alga Volvox carteri in response to external CO2 levels possibly regulated by CCM1/CIA5 ortholog.Photosynthesis research · 2011Article
- Localization of putative carbonic anhydrases in two marine diatoms, Phaeodactylum tricornutum and Thalassiosira pseudonana.Photosynthesis research · 2011Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A single intracellular carbonic anhydrase (CA) was detected in air-grown and, at reduced levels, in high CO(2)-grown cells of the marine diatom Phaeodactylum tricornutum (UTEX 642). No external CA activity was detected irrespective of growth CO(2) conditions. Ethoxyzolamide (0.4 mM), a CA-specific inhibitor, severely inhibited high-affinity photosynthesis at low concentrations of dissolved inorganic carbon, whereas 2 mM acetazolamide had little effect on the affinity for dissolved inorganic carbon, suggesting that internal CA is crucial for the operation of a carbon concentrating mechanism in P. tricornutum. Internal CA was purified 36.7-fold of that of cell homogenates by ammonium sulfate precipitation, and two-step column chromatography on diethylaminoethyl-sephacel and p-aminomethylbenzene sulfone amide agarose. The purified CA was shown, by SDS-PAGE, to comprise an electrophoretically single polypeptide of 28 kD under both reduced and nonreduced conditions. The entire sequence of the cDNA of this CA was obtained by the rapid amplification of cDNA ends method and indicated that the cDNA encodes 282 amino acids. Comparison of this putative precursor sequence with the N-terminal amino acid sequence of the purified CA indicated that it included a possible signal sequence of up to 46 amino acids at the N terminus. The mature CA was found to consist of 236 amino acids and the sequence was homologous to beta-type CAs. Even though the zinc-ligand amino acid residues were shown to be completely conserved, the amino acid residues that may constitute a CO(2)-binding site appeared to be unique among the beta-CAs so far reported.
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