ArticleInfection and immunity2001
Detection of melanin-like pigments in the dimorphic fungal pathogen Paracoccidioides brasiliensis in vitro and during infection.
Article in Infection and immunity, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 123 citations in OpenAlex.
- Dismantling darkness: interdisciplinary perspectives on melanin degradation.World journal of microbiology & biotechnology · 2026Review
- Physicochemical properties, molecular structure, antioxidant activity, and biological function of extracellular melanin fromJournal of Zhejiang University. Science. B · 2022Article
- Dihydroxynaphthalene-Based Allomelanins: A Source of Inspiration for Innovative Technological Materials.ACS omega · 2022Review
- Molecular epidemiology of Paracoccidiodes spp. recovered from patients with paracoccidioidomycosis in a teaching hospital from Minas Gerais State of Brazil.PLoS neglected tropical diseases · 2021Article
- Fungal Melanin and the Mammalian Immune System.Journal of fungi (Basel, Switzerland) · 2021Review
- Fungal immunity and pathogenesis in mammals versus the invertebrate model organism Galleria mellonella.Pathogens and disease · 2021Review
- Bioprocess of Microbial Melanin Production and Isolation.Frontiers in bioengineering and biotechnology · 2021Review
- Beyond Melanin: Proteomics Reveals Virulence-Related Proteins inJournal of fungi (Basel, Switzerland) · 2020Article
- Melanin as a Virulence Factor in Different Species of GenusJournal of fungi (Basel, Switzerland) · 2020Article
- Characterization of a Paracoccidioides spp. strain from southeastern Brazil genotyped as Paracoccidioides restrepiensis (PS3) and review of this phylogenetic species.Genetics and molecular biology · 2020Article
- Functions of fungal melanin beyond virulence.Fungal biology reviews · 2017Article
- Review
- Chromoblastomycosis.Clinical microbiology reviews · 2017Review
- Article
- Identification and Analysis of the Role of Superoxide Dismutases Isoforms in the Pathogenesis of Paracoccidioides spp.PLoS neglected tropical diseases · 2016Article
- The role of melanin pathways in extremotolerance and virulence of Fonsecaea revealed by de novo assembly transcriptomics using illumina paired-end sequencing.Studies in mycology · 2016Article
- Melanin protects Paracoccidioides brasiliensis from the effects of antimicrobial photodynamic inhibition and antifungal drugs.Antimicrobial agents and chemotherapy · 2015Article
- Intracellular growth is dependent on tyrosine catabolism in the dimorphic fungal pathogen Penicillium marneffei.PLoS pathogens · 2015Article
- Effect of Tricyclazole on morphology, virulence and enzymatic alterations in pathogenic fungi Bipolaris sorokiniana for management of spot blotch disease in barley.World journal of microbiology & biotechnology · 2015Article
- Fungal Melanin: What do We Know About Structure?Frontiers in microbiology · 2015Review
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Authors and funding
9 authors at 5 institutions in 5 countries.
Funding
Abstract
Melanins are implicated in the pathogenesis of several human diseases, including some microbial infections. In this study, we analyzed whether the conidia and the yeasts of the thermally dimorphic fungal pathogen Paracoccidioides brasiliensis produce melanin or melanin-like compounds in vitro and during infection. Growth of P. brasiliensis mycelia on water agar alone produced pigmented conidia, and growth of yeasts in minimal medium with L-3,4-dihydroxyphenylalanine (L-DOPA) produced pigmented cells. Digestion of the pigmented conidia and yeasts with proteolytic enzymes, denaturant, and hot concentrated acid yielded dark particles that were the same size and shape as their propagules. Immunofluorescence analysis demonstrated reactivity of a melanin-binding monoclonal antibody (MAb) with the pigmented conidia, yeasts, and particles. Electron spin resonance spectroscopy identified the yeast-derived particles produced in vitro when P. brasiliensis was grown in L-DOPA medium as a melanin-like compound. Nonreducing polyacrylamide gel electrophoresis of cytoplasmic yeast extract revealed a protein that catalyzed melanin synthesis from L-DOPA. The melanin binding MAb reacted with yeast cells in tissue from mice infected with P. brasiliensis. Finally digestion of infected tissue liberated particles reactive to the melanin binding MAb that had the typical morphology of P. brasiliensis yeasts. These data strongly suggest that P. brasiliensis propagules, both conidia and yeast cells, can produce melanin or melanin-like compounds in vitro and in vivo. Based on what is known about the function of melanin in the virulence of other fungi, this pigment may play a role in the pathogenesis of paracoccidioidomycosis.
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