ArticleInfection and immunity2001
dksA is required for intercellular spread of Shigella flexneri via an RpoS-independent mechanism.
Article in Infection and immunity, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- DksA coordinates bile-mediated regulation of virulence-associated phenotypes in type three secretion system-positiveMicrobiology (Reading, England) · 2021Article
- Functional Annotation and Curation of Hypothetical Proteins Present in A Newly Emerged Serotype 1c ofGenes · 2020Article
- Guanosine tetraphosphate relieves the negative regulation of Salmonella pathogenicity island-2 gene transcription exerted by the AT-rich ssrA discriminator region.Scientific reports · 2018Article
- Regulatory Hierarchies Controlling Virulence Gene Expression inFrontiers in microbiology · 2018Review
- Bacterial Survival under Extreme UV Radiation: A Comparative Proteomics Study ofFrontiers in microbiology · 2017Article
- Molecular and Cellular Mechanisms of Shigella flexneri Dissemination.Frontiers in cellular and infection microbiology · 2016Review
- The Stringent Response Regulator DksA Is Required for Salmonella enterica Serovar Typhimurium Growth in Minimal Medium, Motility, Biofilm Formation, and Intestinal Colonization.Infection and immunity · 2016Article
- Article
- pH dependence of the stress regulator DksA.PloS one · 2015Article
- Expression of Shigella flexneri gluQ-rs gene is linked to dksA and controlled by a transcriptional terminator.BMC microbiology · 2012Article
- Functional characterization of the stringent response regulatory gene dksA of Vibrio cholerae and its role in modulation of virulence phenotypes.Journal of bacteriology · 2012Article
- Genetic components of stringent response in Vibrio cholerae.The Indian journal of medical research · 2011Review
- ppGpp conjures bacterial virulence.Microbiology and molecular biology reviews : MMBR · 2010Review
- Role of RpoS in virulence of pathogens.Infection and immunity · 2010Review
- Role of the DksA-like protein in the pathogenesis and diverse metabolic activity of Campylobacter jejuni.Journal of bacteriology · 2008Article
- Complete genome sequence of Shigella flexneri 5b and comparison with Shigella flexneri 2a.BMC genomics · 2006Article
- Transcriptome and physiological responses to hydrogen peroxide of the facultatively phototrophic bacterium Rhodobacter sphaeroides.Journal of bacteriology · 2005Article
- DksA potentiates direct activation of amino acid promoters by ppGpp.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Transcriptional adaptation of Shigella flexneri during infection of macrophages and epithelial cells: insights into the strategies of a cytosolic bacterial pathogen.Infection and immunity · 2005Article
- Posttranscriptional control of quorum-sensing-dependent virulence genes by DksA in Pseudomonas aeruginosa.Journal of bacteriology · 2003Article
Corrections and comments
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Authors and funding
4 authors.
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Abstract
Pathogenesis of Shigella flexneri is dependent on the ability of the bacterium to invade and spread within epithelial cells. In this study, we identified dksA as a gene necessary for intercellular spread in, but not invasion of, cultured cells. The S. flexneri dksA mutant exhibited sensitivity to acid and oxidative stress, in part due to an effect of DksA on production of RpoS. However, an S. flexneri rpoS mutant formed plaques on tissue culture monolayers, thus excluding DksA regulation of RpoS as the mechanism responsible for the inability of the dksA mutant to spread intercellularly. Intracellular analysis of the dksA mutant indicates that it survived and divided within the Henle cell cytoplasm, but the dksA mutant cells were elongated, and some exhibited filamentation in the intracellular environment. Some of the S. flexneri dksA mutant cells showed aberrant localization of virulence protein IcsA, which may inhibit spread between epithelial cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.