ArticleInfection and immunity2001
ADP-ribosylation of Rab5 by ExoS of Pseudomonas aeruginosa affects endocytosis.
Article in Infection and immunity, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 50 citations in OpenAlex.
- Individual contributions of exotoxins S and T on internalizedInfection and immunity · 2026Article
- Evidence for intracellularJournal of bacteriology · 2024Review
- Legionella metaeffector MavL reverses ubiquitin ADP-ribosylation via a conserved arginine-specific macrodomain.Nature communications · 2024Article
- Catheter-associated urinary tract infection byProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Genomic Surveillance of Clinical Pseudomonas aeruginosa Isolates Reveals an Additive Effect of Carbapenemase Production on Carbapenem Resistance.Microbiology spectrum · 2022Article
- The Role ofToxins · 2021Review
- Glyceraldehyde-3-phosphate Dehydrogenase Common Peptides ofVaccines · 2021Article
- Inverse control of Rab proteins byScience advances · 2020Article
- Epitopes for Multivalent Vaccines AgainstFrontiers in cellular and infection microbiology · 2020Article
- Sialic Acid-Siglec-E Interactions DuringFrontiers in immunology · 2020Article
- Pseudomonas aeruginosa ExsA Regulates a Metalloprotease, ImpA, That Inhibits Phagocytosis of Macrophages.Infection and immunity · 2019Article
- The Population Structure of Pseudomonas aeruginosa Is Characterized by Genetic Isolation of exoU+ and exoS+ Lineages.Genome biology and evolution · 2019Article
- MAV_4644 Interaction with the Host Cathepsin Z ProtectsMicroorganisms · 2019Article
- Pseudomonas aeruginosa: breaking down barriers.Current genetics · 2016Review
- Article
- Sequential inactivation of Rho GTPases and Lim kinase by Pseudomonas aeruginosa toxins ExoS and ExoT leads to endothelial monolayer breakdown.Cellular and molecular life sciences : CMLS · 2014Article
- The emerging potential of autophagy-based therapies in the treatment of cystic fibrosis lung infections.Autophagy · 2014Article
- Who's really in control: microbial regulation of protein trafficking in the epithelium.American journal of physiology. Cell physiology · 2014Review
- Exoenzyme S ADP-ribosylates Rab5 effector sites to uncouple intracellular trafficking.Infection and immunity · 2014Article
- In vitro assays to monitor the activity of Pseudomonas aeruginosa Type III secreted proteins.Methods in molecular biology (Clifton, N.J.) · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pseudomonas aeruginosa exoenzyme S (ExoS) is an ADP-ribosyltransferase that modifies low-molecular-weight GTPases. Here we studied the effect of Rab5 ADP-ribosylation by ExoS on its cellular function, i.e., regulation of early endocytic events. Coculture of CHO cells with P. aeruginosa induced a marked decrease in horseradish peroxidase (HRP) uptake compared to noninfected cells, while coculture with a P. aeruginosa mutant strain that fails to produce ExoS did not lead to any change in HRP uptake. Microinjection of recombinant ExoS into Xenopus oocytes induced strong inhibition of basal HRP uptake by oocytes. Moreover, coinjection of recombinant ExoS with Rab5 abolished the typical stimulation of HRP uptake obtained after GTPase microinjection. Cytosols prepared from injected oocytes were used in an endosome-endosome fusion assay. Cytosol from ExoS-microinjected oocytes was ineffective in promoting endosome-endosome fusion. However, in these conditions, the addition of Rab5 to the assay led to fusion recovery. Finally, we found that the interaction of Rab5 with EEA1 was markedly diminished after Rab5 ADP-ribosylation by ExoS.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.