ArticleInfection and immunity2001
Recognition of Lewis x derivatives present on mucins by flagellar components of Pseudomonas aeruginosa.
Article in Infection and immunity, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 95 citations in OpenAlex.
- Glycan-mediated adhesion mechanisms in antibiotic-resistant bacteria.BBA advances · 2025Article
- Insights onMicroorganisms · 2024Article
- Assessment of the Glycan-Binding Profile of Pseudomonas aeruginosa PAO1.Microbiology spectrum · 2023Article
- Assessment of the Glycan-Binding Profile ofbioRxiv : the preprint server for biology · 2023Article
- Interference of flagellar rotation up-regulates the expression of small RNA contributing toScience advances · 2022Article
- Antibacterial mechanism of linalool emulsion against Pseudomonas aeruginosa and its application to cold fresh beef.World journal of microbiology & biotechnology · 2022Article
- Molecular Mechanisms Involved in Pseudomonas aeruginosa Bacteremia.Advances in experimental medicine and biology · 2022Review
- Flagella, Chemotaxis and Surface Sensing.Advances in experimental medicine and biology · 2022Article
- Molecular Determinants of Filament Capping Proteins Required for the Formation of Functional Flagella in Gram-Negative Bacteria.Biomolecules · 2021Article
- Bacterial Flagellar Filament: A Supramolecular Multifunctional Nanostructure.International journal of molecular sciences · 2021Review
- Mucins trigger dispersal ofNPJ biofilms and microbiomes · 2018Article
- 3-Sulfogalactosyl-dependent adhesion ofThe Journal of biological chemistry · 2017Article
- Immune Recognition of the Epidemic Cystic Fibrosis Pathogen Burkholderia dolosa.Infection and immunity · 2017Article
- Review
- Cyclic diguanylate signaling in Gram-positive bacteria.FEMS microbiology reviews · 2016Review
- Bacterial flagella: twist and stick, or dodge across the kingdoms.PLoS pathogens · 2015Review
- Article
- The putative Poc complex controls two distinct Pseudomonas aeruginosa polar motility mechanisms.Molecular microbiology · 2013Article
- Article
- Effect of dietary monosaccharides on Pseudomonas aeruginosa virulence.Surgical infections · 2013Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
Pseudomonas aeruginosa binds to human respiratory mucins by mechanisms involving flagellar component-receptor interactions. The adhesion of P. aeruginosa strain PAK is mediated by the flagellar cap protein, FliD, without the involvement of flagellin. Two distinct types of FliD proteins have been identified in P. aeruginosa: A type, found in strain PAK, and B type, found in strain PAO1. In the present work, studies performed with the P. aeruginosa B-type strain PAO1 indicate that both the FliD protein and the flagellin of this strain are involved in the binding to respiratory mucins. Using polyacrylamide-based fluorescent glycoconjugates in a flow cytometry assay, it was previously demonstrated that P. aeruginosa recognizes Le(x) (or Lewis x) derivatives found at the periphery of human respiratory mucins. The aim of the present work was therefore to determine whether these carbohydrate epitopes (or glycotopes) are receptors for FliD proteins and flagellin. The results obtained by both flow cytometry and a microplate adhesion assay indicate that the FliD protein of strain PAO1 is involved in the binding of glycoconjugates bearing Le(x) or sialyl-Le(x) determinants, while the binding of flagellin is restricted to the glycoconjugate bearing Le(x) glycotope. In contrast, the type A cap protein of P. aeruginosa strain PAK is not involved in the binding to glycoconjugates bearing Le(x), sialyl-Le(x), or sulfosialyl-Le(x) glycotopes. This study demonstrates a clear association between a specific Pseudomonas adhesin and a specific mucin glycotope and demonstrates that fine specificities exist in mucin recognition by P. aeruginosa.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.