ArticleThe EMBO journal2001
HBV infection of cell culture: evidence for multivalent and cooperative attachment.
Article in The EMBO journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 88 citations in OpenAlex.
- Enhanced diffusion through multivalency.Soft matter · 2025Article
- Methylation profile of hepatitis B virus is not influenced by interferon α in human liver cancer cells.Molecular medicine reports · 2021Article
- [A review on cell-based models of human liver disease in vitro].Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi · 2021Review
- Quantification, epitope mapping and genotype cross-reactivity of hepatitis B preS-specific antibodies in subjects vaccinated with different dosage regimens of BM32.EBioMedicine · 2020Article
- Exosomes derived from HBV-associated liver cancer promote chemoresistance by upregulating chaperone-mediated autophagy.Oncology letters · 2019Article
- Investigating the hepatitis B virus life cycle using engineered reporter hepatitis B viruses.Cancer science · 2018Article
- HBV culture and infectious systems.Hepatology international · 2016Review
- TMEM2 inhibits hepatitis B virus infection in HepG2 and HepG2.2.15 cells by activating the JAK-STAT signaling pathway.Cell death & disease · 2016Article
- Review
- New Methods in Tissue Engineering: Improved Models for Viral Infection.Annual review of virology · 2014Article
- Telbivudine treatment corrects HBV-induced epigenetic alterations in liver cells of patients with chronic hepatitis B.Carcinogenesis · 2014Article
- Direct acting antivirals for the treatment of chronic viral hepatitis.Scientifica · 2012Review
- Ferritin light chain and squamous cell carcinoma antigen 1 are coreceptors for cellular attachment and entry of hepatitis B virus.International journal of nanomedicine · 2012Article
- DNA double-strand breaks, potential targets for HBV integration.Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban · 2010Article
- Hepatitis B virus replication induces methylation of both host and viral DNA.Journal of virology · 2010Article
- HBV life cycle: entry and morphogenesis.Viruses · 2009Article
- Two-dimensional blue native/SDS-PAGE analysis reveals heat shock protein chaperone machinery involved in hepatitis B virus production in HepG2.2.15 cells.Molecular & cellular proteomics : MCP · 2009Article
- Replication of clinical hepatitis B virus isolate and its application for selecting antiviral agents for chronic hepatitis B patients.World journal of gastroenterology · 2008Article
- Modulation of iridovirus-induced apoptosis by endocytosis, early expression, JNK, and apical caspase.Virology · 2008Article
- Molecular virology of hepatitis B virus for clinicians.Clinics in liver disease · 2007Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hepadnaviruses do not infect cultured cells, therefore our knowledge of the mechanism of the early stages of virus-cell interaction is rather poor. In this study, we show that dimethylsulfoxide (DMSO)-treated HepG2 hepatoblastoma cells are infected efficiently by serum-derived hepatitis B virus (HBV) as monitored by viral gene expression and replication markers. To measure virus attachment, a variety of HBV surface proteins (HBsAgs) were conjugated to polystyrene beads and their capacity to attach cells was visualized and quantified by light microscopy at a single-cell resolution. Remarkably, DMSO increases the attachment efficiency by >200-fold. We further identify the QLDPAF sequence within preS1 as the receptor-binding viral domain epitope. Interestingly, a similar sequence is shared by several cellular, bacterial and viral proteins involved in cell adhesion, attachment and fusion. We also found that the small HBsAg contains a secondary attachment site that recognizes a distinct receptor on the cell membrane. Furthermore, we provide evidence in support of multivalent HBV attachment with synergistic interplay. Our data depict a mechanistic view of virus attachment and ingestion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.