ArticleCancer gene therapy2001
Cathepsin L antisense oligonucleotides in a human osteosarcoma cell line: effects on the invasive phenotype.
Article in Cancer gene therapy, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 57 citations in OpenAlex.
- Cathepsin L promotes oesophageal squamous cell carcinoma development and may be associated with tumour-associated macrophages.Heliyon · 2024Article
- Peripheral Blood Mononuclear Cell Biomarkers for Major Depressive Disorder: A Transcriptomic Approach.Depression and anxiety · 2024Article
- Large-scale whole exome sequencing studies identify two genes,CTSL and APOE, associated with lung cancer.PLoS genetics · 2023Article
- Identification of Factors Driving Doxorubicin-Resistant Ewing Tumor Cells to Survival.Cancers · 2022Article
- CTLA-2 Alpha Is a Potent Inhibitor of Angiogenesis in Murine Ocular Tissue.Antioxidants (Basel, Switzerland) · 2021Article
- Article
- Cystatin C deficiency suppresses tumor growth in a breast cancer model through decreased proliferation of tumor cells.Oncotarget · 2017Article
- Cathepsin L targeting in cancer treatment.Pharmacology & therapeutics · 2015Review
- Acquired resistance to metformin in breast cancer cells triggers transcriptome reprogramming toward a degradome-related metastatic stem-like profile.Cell cycle (Georgetown, Tex.) · 2014Article
- Cysteine cathepsins: from structure, function and regulation to new frontiers.Biochimica et biophysica acta · 2012Review
- Cathepsin L increased level upon Ras mutants expression: the role of p38 and p44/42 MAPK signaling pathways.Molecular and cellular biochemistry · 2010Article
- Frequent loss of cystatin E/M expression implicated in the progression of prostate cancer.Oncogene · 2009Article
- The cytoplasmic domain of proEGF negatively regulates motility and elastinolytic activity in thyroid carcinoma cells.Neoplasia (New York, N.Y.) · 2008Article
- Article
- Cathepsin L increases invasion and migration of B16 melanoma.Cancer cell international · 2007Article
- Relaxin enhances the oncogenic potential of human thyroid carcinoma cells.The American journal of pathology · 2006Article
- The importance of cysteine cathepsin proteases for placental development.Journal of molecular medicine (Berlin, Germany) · 2006Article
- Expression of cathepsin L in human tumor cells is under the control of distinct regulatory mechanisms.Oncogene · 2006Article
- Article
- Establishment and characterization of the permanent human cell line C3842 derived from a secondary chondrosarcoma in Ollier's disease.Virchows Archiv : an international journal of pathology · 2005Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Alterations in cathepsin L expression and trafficking have been associated with the progression and metastasis of several tumor entities. In the present study, we examined the effects of various cathepsin L antisense (as) phosphorothioate oligonucleotides on both the expression of cathepsin L and the invasive potential of the human osteosarcoma cell line MNNG/HOS. Seven oligonucleotides of 20-bp length each and one random control oligonucleotide were chosen to block cathepsin L expression. Northern blot analysis demonstrated a significant reduction in cathepsin L mRNA expression by the six antisense oligonucleotides at a concentration of 10 microM. Cathepsin L protein expression was reduced significantly (50-85%) by the antisense oligonucleotides, as compared with the controls. Adhesion to matrices of collagen I and matrigel was not affected. In in vitro motility and invasion assays performed in uncoated and precoated transwell chambers, the ability of cells to migrate through the filters was inhibited by 35-75% using antisense oligonucleotides. The random control did not show any inhibitory effect. These data demonstrate that in MNNG/HOS cells cathepsin L influences cellular malignancy by promoting migration and basement membrane degradation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.