ArticleRNA (New York, N.Y.)2001
Ribosomal protein L5 helps anchor peptidyl-tRNA to the P-site in Saccharomyces cerevisiae.
Article in RNA (New York, N.Y.), 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed, 56 citations in OpenAlex.
- Comprehensive Proteome and Acetyl-Proteome Atlas Reveals Hepatic Lipid Metabolism in Layer Hens with Fatty Liver Hemorrhagic Syndrome.International journal of molecular sciences · 2023Article
- Molecular characterization and expression analysis ofSaudi journal of biological sciences · 2021Article
- Review
- Multi-protein bridging factor 1(Mbf1), Rps3 and Asc1 prevent stalled ribosomes from frameshifting.eLife · 2018Article
- Translation Elongation and Recoding in Eukaryotes.Cold Spring Harbor perspectives in biology · 2018Review
- Global shape mimicry of tRNA within a viral internal ribosome entry site mediates translational reading frame selection.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- A large-scale functional screen identifies Nova1 and Ncoa3 as regulators of neuronal miRNA function.The EMBO journal · 2015Article
- Functions of ribosomal proteins in assembly of eukaryotic ribosomes in vivo.Annual review of biochemistry · 2015Review
- Eukaryotic rpL10 drives ribosomal rotation.Nucleic acids research · 2014Article
- Insights into the mechanism of ribosomal incorporation of mammalian L13a protein during ribosome biogenesis.Molecular and cellular biology · 2013Article
- Yeast ribosomal protein L40 assembles late into precursor 60 S ribosomes and is required for their cytoplasmic maturation.The Journal of biological chemistry · 2012Article
- A rapid, inexpensive yeast-based dual-fluorescence assay of programmed--1 ribosomal frameshifting for high-throughput screening.Nucleic acids research · 2011Article
- Five proteins of Laodelphax striatellus are potentially involved in the interactions between rice stripe virus and vector.PloS one · 2011Article
- Transmembrane receptor DCC associates with protein synthesis machinery and regulates translation.Cell · 2010Article
- Achieving a golden mean: mechanisms by which coronaviruses ensure synthesis of the correct stoichiometric ratios of viral proteins.Journal of virology · 2010Article
- The eukaryotic ribosome: current status and challenges.The Journal of biological chemistry · 2009Review
- Yeast ribosomal protein L10 helps coordinate tRNA movement through the large subunit.Nucleic acids research · 2008Article
- Functional features of the C-terminal region of yeast ribosomal protein L5.Molecular genetics and genomics : MGG · 2008Article
- A new kinetic model reveals the synergistic effect of E-, P- and A-sites on +1 ribosomal frameshifting.Nucleic acids research · 2008Article
- Different mechanisms for pseudouridine formation in yeast 5S and 5.8S rRNAs.Molecular and cellular biology · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Our previous demonstration that mutants of 5S rRNA called mof9 can specifically alter efficiencies of programmed ribosomal frameshifting (PRF) suggested a role for this ubiquitous molecule in the maintenance of translational reading frame, though the repetitive nature of the 5S rDNA gene (>100 copies/cell) inhibited more detailed analyses. However, given the known interactions between 5S rRNA and ribosomal protein L5 (previously called L1 or YL3) encoded by an essential, single-copy gene, we monitored the effects of a series of well-defined rpl5 mutants on PRF and virus propagation. Consistent with the mof9 results, we find that the rpl5 mutants promoted increased frameshifting efficiencies in both the -1 and +1 directions, and conferred defects in the ability of cells to propagate two endogenous viruses. Biochemical analyses demonstrated that mutant ribosomes had decreased affinities for peptidyl-tRNA. Pharmacological studies showed that sparsomycin, a peptidyltransferase inhibitor that specifically increases the binding of peptidyl-tRNA with ribosomes, was antagonistic to the frameshifting defects of the most severe mutant, and the extent of sparsomycin resistance correlated with the severity of the frameshifting defects in all of the mutants. These results provide biochemical and physiological evidence that one function of L5 is to anchor peptidyl-tRNA to the P-site. A model is presented describing how decreased affinity of ribosomes for peptidyl-tRNA can affect both -1 and +1 frameshifting, and for the effects of sparsomycin.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.