ArticlePharmaceutical research2001
Protamine sulfate enhances the transduction efficiency of recombinant adeno-associated virus-mediated gene delivery.
Article in Pharmaceutical research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 37 citations in OpenAlex.
- Adenovector-mediated gene delivery to human umbilical cord mesenchymal stromal cells induces inner ear cell phenotype.Cellular reprogramming · 2013Trial
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- Membrane Filtration of Nanoscale Biomaterials: Model System and Membrane Performance Evaluation for AAV2 Viral Vector Clarification and Recovery.Nanomaterials (Basel, Switzerland) · 2025Article
- Evaluation of drug delivery vehicles for improved transduction of oncolytic adenoviruses in solid tumor tissue.Upsala journal of medical sciences · 2025Article
- Chimeric antigen receptor-based natural killer cell immunotherapy in cancer: from bench to bedside.Cell death & disease · 2024Review
- Novel gene manipulation approaches to unlock the existing bottlenecks of CAR-NK cell therapy.Frontiers in cell and developmental biology · 2024Review
- Methodological Approaches for Increasing the Retroviral Transduction Efficiency of Primary NK Cells.Current pharmaceutical design · 2024Article
- CAR-NK Cells: From Natural Basis to Design for Kill.Frontiers in immunology · 2021Review
- Development of anMolecular therapy. Methods & clinical development · 2020Article
- Rosuvastatin Enhances VSV-G Lentiviral Transduction of NK Cells via Upregulation of the Low-Density Lipoprotein Receptor.Molecular therapy. Methods & clinical development · 2020Article
- Commentary: A Cost-Effective Method to Enhance Adenoviral Transduction of Primary Murine Osteoblasts and Bone Marrow Stromal Cells.Frontiers in endocrinology · 2020Article
- A new chemical complex can rapidly concentrate lentivirus and significantly enhance gene transduction.Cytotechnology · 2018Article
- Efficient lentiviral transduction of human mesenchymal stem cells that preserves proliferation and differentiation capabilities.Stem cells translational medicine · 2012Article
- Article
- Glucose- and metabolically regulated hepatic insulin gene therapy for diabetes.Pharmaceutical research · 2008Article
- Glucose-modulated transgene expression via recombinant adeno-associated virus.Pharmaceutical research · 2002Article
- Optimization of lentiviral transduction parameters and its application for CRISPR-based secretome modification of human endometrial mesenchymal stem cells.Cell cycle (Georgetown, Tex.)Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThe purpose of this study was to evaluate glucose responsiveness in HepG2 human hepatoma cells transduced by a recombinant adeno-associated virus (rAAV) vector containing the insulin gene promoter. and to investigate the effect of protamine sulfate on rAAV-mediated gene delivery.
methodsRecombinant AAV vector, AAV.Ins.Luc.delta EGFP, was employed to transduce HepG2 hepatoma cells. Virus infection was carried out either in the absence or presence of protamine sulfate, followed by fluorescence microscopic examination, luciferase activity assay, and flow cytometric analysis. Electrokinetic measurements were carried out to determine the effect of protamine sulfate on zeta potential of the cells and the virus.
resultsGlucose-responsive luciferase gene expression was obtained in rAAV-transduced HepG2 cells. Addition of 5 microg/ml protamine reversed the zeta potential of the cells and the virus particles, leading to enhanced transgene expression in rAAV-transduced HepG2 cells. Enhancement of protamine sulfate on rAAV-mediated gene transfer was dose-dependent. Addition of more than 5 microg/ml protamine resulted in a reduction of infectability of the virus.
conclusionsGlucose responsiveness in the millimolar concentration range can be obtained in rAAV-transduced HepG2 cells. Protamine sulfate, up to 5 microg/ml, enhanced the rAAV transduction efficiency in HepG2 cells. The enhancement was correlated with zeta potential of the cells and the virus.
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