ArticleThe Journal of experimental medicine2001
Quantitative regulation of class switch recombination by switch region transcription.
Article in The Journal of experimental medicine, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed.
- Lineage tracing reveals fate bias and transcriptional memory in human B cells.Life science alliance · 2023Article
- B cells defined by immunoglobulin isotypes.Clinical and experimental immunology · 2022Article
- DSB structure impacts DNA recombination leading to class switching and chromosomal translocations in human B cells.PLoS genetics · 2019Article
- DNA damage and genome instability by G-quadruplex ligands are mediated by R loops in human cancer cells.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Molecular regulation of peripheral B cells and their progeny in immunity.Genes & development · 2019Review
- R-Loop Depletion by Over-expressed RNase H1 in Mouse B Cells Increases Activation-Induced Deaminase Access to the Transcribed Strand without Altering Frequency of Isotype Switching.Journal of molecular biology · 2017Article
- RNA Exosome Regulates AID DNA Mutator Activity in the B Cell Genome.Advances in immunology · 2015Review
- Topoisomerase I plays a critical role in suppressing genome instability at a highly transcribed G-quadruplex-forming sequence.PLoS genetics · 2014Article
- Decrease in topoisomerase I is responsible for activation-induced cytidine deaminase (AID)-dependent somatic hypermutation.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Cathelin-related antimicrobial peptide differentially regulates T- and B-cell function.European journal of immunology · 2011Article
- Overlapping activation-induced cytidine deaminase hotspot motifs in Ig class-switch recombination.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Sense transcription through the S region is essential for immunoglobulin class switch recombination.The EMBO journal · 2011Article
- Single nucleotide changes in the human Igamma1 and Igamma4 promoters underlie different transcriptional responses to CD40.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
- Augmentation of antibody responses by retinoic acid and costimulatory molecules.Seminars in immunology · 2009Review
- Requirement for enhancer specificity in immunoglobulin heavy chain locus regulation.Journal of immunology (Baltimore, Md. : 1950) · 2008Article
- AID to overcome the limitations of genomic information by introducing somatic DNA alterations.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2006Review
- Role of Id proteins in B lymphocyte activation: new insights from knockout mouse studies.Journal of molecular medicine (Berlin, Germany) · 2004Review
- Synergistic interaction of two independent genetic loci causes extreme elevation of serum IgA in mice.Genes and immunity · 2004Article
- Somatic hypermutation is limited by CRM1-dependent nuclear export of activation-induced deaminase.The Journal of experimental medicine · 2004Article
- Mutations occur in the Ig Smu region but rarely in Sgamma regions prior to class switch recombination.The EMBO journal · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
The isotype specificity of immunoglobulin (Ig) class switching is regulated by a cytokine which induces transcription of a specific switch (S) region, giving rise to so-called germline transcripts. Although previous studies have demonstrated that germline transcription of an S region is required for class switch recombination (CSR) of that particular S region, it has not been shown whether the level of S region transcription affects the efficiency of CSR. We addressed this question by using an artificial DNA construct containing a constitutively transcribed mu switch (Smu) region and an alpha switch (Salpha) region driven by a tetracycline-responsive promoter. The construct was introduced into a switch-inducible B lymphoma line and the quantitative correlation between Salpha region transcription and class switching efficiency was evaluated. The level of Salpha transcription was linearly correlated with CSR efficiency, reaching a plateau at saturation. On the other hand, we failed to obtain the evidence to support involvement of either RNA-DNA heteroduplex or trans germline transcripts in CSR. Taken together, it is likely that S region transcription and/or transcript processing in situ may be required for CSR. We propose that because of the unusual properties of S region DNA, transcription induces the DNA to transiently be single stranded, permitting secondary structure(s) to form. Such structures may be recognition targets of a putative class switch recombinase.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.