ArticleJournal of bacteriology2001
Insertional mutagenesis in the n-alkane-assimilating yeast Yarrowia lipolytica: generation of tagged mutations in genes involved in hydrophobic substrate utilization.
Article in Journal of bacteriology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 100 citations in OpenAlex.
- Article
- Metabolic engineering ofFrontiers in bioengineering and biotechnology · 2025Article
- Article
- A unique, newly discovered four-member protein family involved in extracellular fatty acid binding in Yarrowia lipolytica.Microbial cell factories · 2022Article
- Application of a New Engineered Strain ofInternational journal of molecular sciences · 2021Article
- Sugar versus fat: elimination of glycogen storage improves lipid accumulation in Yarrowia lipolytica.FEMS yeast research · 2017Article
- Sustainable source of omega-3 eicosapentaenoic acid from metabolically engineered Yarrowia lipolytica: from fundamental research to commercial production.Applied microbiology and biotechnology · 2015Review
- Awakening the endogenous Leloir pathway for efficient galactose utilization by Yarrowia lipolytica.Biotechnology for biofuels · 2015Article
- Dynein light chain interaction with the peroxisomal import docking complex modulates peroxisome biogenesis in yeast.Journal of cell science · 2013Article
- Transporter engineering for improved tolerance against alkane biofuels in Saccharomyces cerevisiae.Biotechnology for biofuels · 2013Article
- Steroid biotransformations in biphasic systems with Yarrowia lipolytica expressing human liver cytochrome P450 genes.Microbial cell factories · 2012Article
- Influence of glucose and saturated free-fatty acid mixtures on citric acid and lipid production by Yarrowia lipolytica.Current microbiology · 2006Article
- Trs85 is required for macroautophagy, pexophagy and cytoplasm to vacuole targeting in Yarrowia lipolytica and Saccharomyces cerevisiae.Autophagy · 2005Article
- Lipid accumulation, lipid body formation, and acyl coenzyme A oxidases of the yeast Yarrowia lipolytica.Applied and environmental microbiology · 2004Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tagged mutants affected in the degradation of hydrophobic compounds (HC) were generated by insertion of a zeta-URA3 mutagenesis cassette (MTC) into the genome of a zeta-free and ura3 deletion-containing strain of Yarrowia lipolytica. MTC integration occurred predominantly at random by nonhomologous recombination. A total of 8,600 Ura(+) transformants were tested by replica plating for (i) growth on minimal media with alkanes of different chain lengths (decane, dodecane, and hexadecane), oleic acid, tributyrin, or ethanol as the C source and (ii) colonial defects on different glucose-containing media (YPD, YNBD, and YNBcas). A total of 257 mutants were obtained, of which about 70 were affected in HC degradation, representing different types of non-alkane-utilizing (Alk(-)) mutants (phenotypic classes alkA to alkE) and tributyrin degradation mutants. Among Alk(-) mutants, growth defects depending on the alkane chain length were observed (alkAa to alkAc). Furthermore, mutants defective in yeast-hypha transition and ethanol utilization and selected auxotrophic mutants were isolated. Flanking borders of the integrated MTC were sequenced to identify the disrupted genes. Sequence analysis indicated that the MTC was integrated in the LEU1 locus in N083, a leucine-auxotrophic mutant, in the isocitrate dehydrogenase gene of N156 (alkE leaky), in the thioredoxin reductase gene in N040 (alkAc), and in a peroxine gene (PEX14) in N078 (alkD). This indicates that MTC integration is a powerful tool for generating and analyzing tagged mutants in Y. lipolytica.
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