ArticleJournal of bacteriology2001
Characterization of PitA and PitB from Escherichia coli.
Article in Journal of bacteriology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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Who cites it
70 citing papers in PubMed, 143 citations in OpenAlex.
- The removal of iron and phosphate from culture medium by Rhodococcus ruber SiAl.Folia microbiologica · 2026Article
- Toward Solar-Powered Growth of AutotrophicJournal of the American Chemical Society · 2026Article
- DrPitA-mediated enrichment of intracellular manganese and phosphate contributes to oxidative stress resistance ofApplied and environmental microbiology · 2026Article
- Candidatus Dermatophostum as a novel genus of polyphosphate-accumulating organisms for high-strength wastewater treatment.The ISME journal · 2026Article
- Evolved populations ofMicrobial genomics · 2026Article
- Roles of Pho regulon in bacterial pathogenicity.Virulence · 2025Review
- A flow equilibrium model controlling cytoplasmic transition metal cation pools and preventing mis-metalation as exemplified for zinc homeostasis.Journal of bacteriology · 2025Review
- Impeding pathways of intrinsic resistance in Escherichia coli confers antibiotic sensitization and resistance proofing.PLoS biology · 2025Article
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- Context-dependent change in the fitness effect of (in)organic phosphate antiporter glpT during Salmonella Typhimurium infection.Nature communications · 2025Article
- Unveiling the role of the PhoP master regulator in arsenite resistance throughCurrent research in microbial sciences · 2025Article
- OftenFrontiers in microbiology · 2025Article
- The cytoplasmic phosphate level has a central regulatory role in the phosphate starvation response of Caulobacter crescentus.Communications biology · 2024Article
- Chemical Reaction Models in Synthetic Promoter Design in Bacteria.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Phosphorus starvation response and PhoB-independent utilization of organic phosphate sources byMicrobiology spectrum · 2023Article
- xenoGI 3: using the DTLOR model to reconstruct the evolution of gene families in clades of microbes.BMC bioinformatics · 2023Article
- Article
- Genome-wide mapping of thebioRxiv : the preprint server for biology · 2023Article
- Pseudomonas aeruginosa Phosphate Transporter PitA (PA4292) Controls Susceptibility to Aminoglycoside Antibiotics by Regulating the Proton Motive Force.Antimicrobial agents and chemotherapy · 2022Article
- Schindler's legacy: from eutrophic lakes to the phosphorus utilization strategies of cyanobacteria.FEMS microbiology reviews · 2022Review
10 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Escherichia coli contains two major systems for transporting inorganic phosphate (P(i)). The low-affinity P(i) transporter (pitA) is expressed constitutively and is dependent on the proton motive force, while the high-affinity Pst system (pstSCAB) is induced at low external P(i) concentrations by the pho regulon and is an ABC transporter. We isolated a third putative P(i) transport gene, pitB, from E. coli K-12 and present evidence that pitB encodes a functional P(i) transporter that may be repressed at low P(i) levels by the pho regulon. While a pitB(+) cosmid clone allowed growth on medium containing 500 microM P(i), E. coli with wild-type genomic pitB (pitA Delta pstC345 double mutant) was unable to grow under these conditions, making it indistinguishable from a pitA pitB Delta pstC345 triple mutant. The mutation Delta pstC345 constitutively activates the pho regulon, which is normally induced by phosphate starvation. Removal of pho regulation by deleting the phoB-phoR operon allowed the pitB(+) pitA Delta pstC345 strain to utilize P(i), with P(i) uptake rates significantly higher than background levels. In addition, the apparent K(m) of PitB decreased with increased levels of protein expression, suggesting that there is also regulation of the PitB protein. Strain K-10 contains a nonfunctional pitA gene and lacks Pit activity when the Pst system is mutated. The pitA mutation was identified as a single base change, causing an aspartic acid to replace glycine 220. This mutation greatly decreased the amount of PitA protein present in cell membranes, indicating that the aspartic acid substitution disrupts protein structure.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.