ArticlePlant molecular biology2001
Characterization of two thioredoxins h with predominant localization in the nucleus of aleurone and scutellum cells of germinating wheat seeds.
Article in Plant molecular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 77 citations in OpenAlex.
- Understanding the Role of Durum Wheat ThioredoxinPlants (Basel, Switzerland) · 2026Article
- Article
- Regulation of seed germination: ROS, epigenetic, and hormonal aspects.Journal of advanced research · 2025Review
- Article
- Thioredoxin TRXo1 is involved in ABA perception via PYR1 redox regulation.Redox biology · 2023Article
- Hydrogen peroxide-induced stress acclimation in plants.Cellular and molecular life sciences : CMLS · 2022Review
- Reactive Oxygen Species (ROS) and Nucleic Acid Modifications During Seed Dormancy.Plants (Basel, Switzerland) · 2020Review
- The expression pattern of OsDim1 in rice and its proposed function.Scientific reports · 2019Article
- Thiol Based Redox Signaling in Plant Nucleus.Frontiers in plant science · 2018Review
- Thioredoxin (Trxo1) interacts with proliferating cell nuclear antigen (PCNA) and its overexpression affects the growth of tobacco cell culture.Redox biology · 2017Article
- Over-expression of Trxo1 increases the viability of tobacco BY-2 cells under H2O2 treatment.Annals of botany · 2015Article
- A cytosolic thioredoxin acts as a molecular chaperone for peroxisome matrix proteins as well as antioxidant in peroxisome.Molecules and cells · 2015Article
- Dissecting the integrative antioxidant and redox systems in plant mitochondria. Effect of stress and S-nitrosylation.Frontiers in plant science · 2013Article
- NADPH thioredoxin reductase C is localized in plastids of photosynthetic and nonphotosynthetic tissues and is involved in lateral root formation in Arabidopsis.The Plant cell · 2012Article
- OsTRXh1 regulates the redox state of the apoplast and influences stress responses in rice.Plant signaling & behavior · 2012Article
- An apoplastic h-type thioredoxin is involved in the stress response through regulation of the apoplastic reactive oxygen species in rice.Plant physiology · 2011Article
- A comparative analysis of the NADPH thioredoxin reductase C-2-Cys peroxiredoxin system from plants and cyanobacteria.Plant physiology · 2011Article
- Identification and characterization of thioredoxin h isoforms differentially expressed in germinating seeds of the model legume Medicago truncatula.Plant physiology · 2011Article
- Mitochondrial and nuclear localization of a novel pea thioredoxin: identification of its mitochondrial target proteins.Plant physiology · 2009Article
- A hydrogen peroxide detoxification system in the nucleus of wheat seed cells: protection or signaling role?Plant signaling & behavior · 2009Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two full-length cDNA clones, designated TrxhA and TrxhB, encoding different but very similar thioredoxin h polypeptides were isolated from wheat (Triticum aestivum cv. Chinese Spring) aleurone cells. The deduced proteins show a high similarity to each other and to thioredoxin h from other sources, in particular from T. aestivum and T. durum. One of them, TRXhA, was expressed in E. coli as a His-tagged polypeptide and used to raise polyclonal antibodies by immunization of rabbits. These antibodies identified a single band (ca. 13.5 kDa) in western blot analysis of protein extracts from all wheat organs analyzed. TRXhA and TRXhB when expressed in E. coli as intact polypetides showed indistinguishable electrophoretic mobility, which corresponded to the 13.5 kDa polypeptide detected in wheat protein extracts. The amount of thioredoxin h transcripts increased in scutellum and aleurone cells during germination but GA3 did not exert any stimulatory effect on thioredoxin h expression. Although northern blot analysis detected a single band, competitive RT-PCR showed that this band is due to the accumulation of both TrxhA and TrxhB mRNAs. These results suggest that the single band detected in western blots is due to the presence of at least two thioredoxin h polypeptides. Immunolocalization experiments confirmed the high content of thioredoxins h in scutellum and aleurone cells, and showed a low content in the starchy endosperm of germinating grains. Interestingly, though these proteins are evenly distributed in the cytosol, the highest levels of thioredoxins h were detected in the nucleus, both in aleurone and scutellum cells.
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