ArticlePlant molecular biology2001
A Kunitz trypsin inhibitor gene family from trembling aspen (Populus tremuloides Michx.): cloning, functional expression, and induction by wounding and herbivory.
Article in Plant molecular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Divergent functions of three Kunitz trypsin inhibitor (KTI) proteins in herbivore defense in poplar.BMC plant biology · 2025Article
- Integrated Analysis of microRNA and mRNA Transcriptome Reveals the Molecular Mechanism ofFrontiers in microbiology · 2021Article
- Defense Priming inPlants (Basel, Switzerland) · 2020Article
- Host plant genetic control of associated fungal and insect species in aEcology and evolution · 2020Article
- Specificity of Herbivore Defense Responses in a Woody Plant, Black Poplar (Populus nigra).Journal of chemical ecology · 2019Article
- Suppression of Plant Defenses by Herbivorous Mites Is Not Associated with Adaptation to Host Plants.International journal of molecular sciences · 2018Article
- The Mexican bean beetle (Epilachna varivestis) regurgitome and insights into beetle-borne virus specificity.PloS one · 2018Article
- Article
- Acquiring nutrients from tree leaves: effects of leaf maturity and development type on a generalist caterpillar.Oecologia · 2017Article
- Influence of Genotype, Environment, and Gypsy Moth Herbivory on Local and Systemic Chemical Defenses in Trembling Aspen (Populus tremuloides).Journal of chemical ecology · 2015Article
- Article
- Molecular characterization of quinate and shikimate metabolism in Populus trichocarpa.The Journal of biological chemistry · 2014Article
- Importance of protein quality versus quantity in alternative host plants for a leaf-feeding insect.Oecologia · 2013Article
- Stress inducible proteinase inhibitor diversity in Capsicum annuum.BMC plant biology · 2012Article
- Overexpression of poplar wounding-inducible genes in Arabidopsis caused improved resistance against Helicoverpa armigera (Hübner) larvae.Breeding science · 2012Article
- Article
- Thaumatin-like proteins are differentially expressed and localized in phloem tissues of hybrid poplar.BMC plant biology · 2010Article
- The wound-, pathogen-, and ultraviolet B-responsive MYB134 gene encodes an R2R3 MYB transcription factor that regulates proanthocyanidin synthesis in poplar.Plant physiology · 2009Article
- Functional analysis of the Kunitz trypsin inhibitor family in poplar reveals biochemical diversity and multiplicity in defense against herbivores.Plant physiology · 2008Article
- Plant defense priming against herbivores: getting ready for a different battle.Plant physiology · 2008Review
Corrections and comments
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Authors and funding
5 authors.
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Abstract
Three Kunitz trypsin inhibitor genes were isolated from trembling aspen (Populus tremuloides) by PCR and cDNA screening. Based on sequence similarity, they were grouped into two classes. Southern blots showed complex banding patterns and a high level of restriction fragment polymorphism between different aspen genotypes, suggesting that these trypsin inhibitors are members of a large, rapidly evolving gene family. One of the trypsin inhibitor genes, PtTI2. was over-expressed in Escherichia coli and its product shown to inhibit bovine trypsin in vitro. Both classes of PtTI genes are induced by wounding and herbivory, permitting rapid adaptive responses to herbivore pressure. The response appears to be mediated by an octadecanoid-based signaling pathway, as methyl jasmonate treatments induced the trypsin inhibitors. Wound-induced accumulation of trypsin inhibitor protein was also observed by western blot analysis. The pattern of expression, the apparent rapid evolution of TI genes, and the in vitro trypsin inhibitory activity are consistent with a role in herbivore defense. This work establishes the presence of a functional protein-based inducible defense system in trembling aspen.
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