ArticlePlant molecular biology2001
The 3' non-coding region of a C4 photosynthesis gene increases transgene expression when combined with heterologous promoters.
Article in Plant molecular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 37 citations in OpenAlex.
- Harnessing natural variation for photosynthetic improvement in next-generation crop breeding.Journal of integrative plant biology · 2026Review
- Plant terminators: the unsung heroes of gene expression.Journal of experimental botany · 2023Review
- A bipartite transcription factor module controlling expression in the bundle sheath of Arabidopsis thaliana.Nature plants · 2020Article
- Plant 3' Regulatory Regions From mRNA-Encoding Genes and Their Uses to Modulate Expression.Frontiers in plant science · 2020Review
- An intronless form of the tobacco extensin gene terminator strongly enhances transient gene expression in plant leaves.Plant molecular biology · 2018Article
- An optimized sericin-1 expression system for mass-producing recombinant proteins in the middle silk glands of transgenic silkworms.Transgenic research · 2013Article
- Evaluation of seed storage protein gene 3'-untranslated regions in enhancing gene expression in transgenic rice seed.Transgenic research · 2012Article
- Unique and overlapping expression patterns among members of photosynthesis-associated nuclear gene families in Arabidopsis.Plant physiology · 2008Article
- Untranslated regions from C4 amaranth AhRbcS1 mRNAs confer translational enhancement and preferential bundle sheath cell expression in transgenic C4 Flaveria bidentis.Plant physiology · 2004Article
- The evolution of CThe New phytologist · 2004Review
- Quantitative regulation of the Flaveria Me1 gene is controlled by the 3'-untranslated region and sequences near the amino terminus.Plant molecular biology · 2001Article
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2 authors at 1 institution in 1 country.
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Abstract
The Me1 gene of the dicot Flaveria bidentis encodes NADP malic enzyme, which catalyses the decarboxylation reaction of C4 photosynthesis in bundle sheath cells. We have previously shown that the 3' non-coding region (Me1 3') controls quantitative expression of the gene. We wondered whether Me1 3' can increase expression when combined with heterologous promoters. We tested a highly expressed, constitutive promoter, the S4 promoter from subterranean clover stunt virus, and a highly expressed, leaf-specific promoter, the light-harvesting chlorophyll a/b-binding protein gene 3 (Lhcb3) promoter of Arabidopsis thaliana. Promoter-3'-end combinations were tested in transgenic C4 Flaveria plants and C3 tobacco. We found that Me1 3' increased expression of the gusA reporter gene several-fold in leaves of both species in combination with either of the promoters. In both cases Me1 3' does not alter the expression pattern for either promoter. We conclude that Me1 3' can be used as a transcription terminator to increase transgene expression in C3 dicot plants.
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