ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2001
Myosin IIB is required for growth cone motility.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 107 papers.
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Who cites it
107 citing papers in PubMed, 223 citations in OpenAlex.
- Differential activity of nonmuscle myosin IIA and IIB isoforms generates a dynamic actomyosin network in a concentration-dependent manner.The Journal of biological chemistry · 2026Article
- Regionalized regulation of actomyosin organization influences cardiomyocyte cell shape changes during chamber curvature formation.Nature communications · 2026Article
- Did axons evolve by activating cytokinesis during interphase? A hypothesis on the origin of neurons.Molecular biology of the cell · 2025Review
- Myosin 2 - A general contractor for the cytoskeleton.Current opinion in cell biology · 2025Review
- β-catenin-α-catenin and actomyosin signaling differentially regulate growth cone contours and axon undulation and branching of retinal ganglion cellsFrontiers in cellular neuroscience · 2025Article
- Myosin II tension sensors visualize force generation within the actin cytoskeleton in living cells.Journal of cell science · 2024Article
- Synapses without tension fail to fire in an in vitro network of hippocampal neurons.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Bayesian traction force estimation using cell boundary-dependent force priors.Biophysical journal · 2023Article
- Article
- Non-Muscle Myosin II in Axonal Cell Biology: From the Growth Cone to the Axon Initial Segment.Cells · 2020Review
- Mechanisms of Axon Elongation Following CNS Injury: What Is Happening at the Axon Tip?Frontiers in cellular neuroscience · 2020Article
- Mechanotransduction in neuronal cell development and functioning.Biophysical reviews · 2019Review
- Myosin IIA and formin dependent mechanosensitivity of filopodia adhesion.Nature communications · 2019Article
- Common and Specific Functions of Nonmuscle Myosin II Paralogs in Cells.Biochemistry. Biokhimiia · 2018Review
- Mammalian nonmuscle myosin II comes in three flavors.Biochemical and biophysical research communications · 2018Review
- Primary Cilia Reconsidered in the Context of Ciliopathies: Extraciliary and Ciliary Functions of Cilia Proteins Converge on a Polarity theme?BioEssays : news and reviews in molecular, cellular and developmental biology · 2018Review
- An Integrated Cytoskeletal Model of Neurite Outgrowth.Frontiers in cellular neuroscience · 2018Review
- Identifying the dynamics of actin and tubulin polymerization in iPSCs and in iPSC-derived neurons.Oncotarget · 2017Article
- Neurite elongation is highly correlated with bulk forward translocation of microtubules.Scientific reports · 2017Article
- Modeling molecular mechanisms in the axon.Computational mechanics · 2017Article
47 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Growth cones are required for the forward advancement and navigation of growing axons. Modulation of growth cone shape and reorientation of the neurite are responsible for the change of outgrowth direction that underlies navigation. Change of shape involves the reordering of the cytoskeleton. Reorientation of the neurite requires the generation of tension, which is supplied by the ability of the growth cone to crawl on a substrate. The specific molecular mechanisms responsible for these activities are unknown but are thought to involve actomyosin-generated force combined with linkage to the cell surface receptors that are responsible for adhesion (Heidemann and Buxbaum, 1998). To test whether myosin IIB is responsible for the force generation, we quantified shape dynamics and filopodial-mediated traction force in growth cones from myosin IIB knock-out (KO) mice and compared them with neurons from normal littermates. Growth cones from the KO mice spread less, showed alterations in shape dynamics and actin organization, and had reduced filopodial-mediated traction force. Although peak traction forces produced by filopodia of KO cones were decreased significantly, KO filopodia occasionally developed forces equivalent to those in the wild type. This indicates that other myosins participate in filopodial-dependent traction force. Therefore, myosin IIB is necessary for normal growth cone spreading and the modulation of shape and traction force but acts in combination with other myosins for some or all of these activities. These activities are essential for growth cone forward advancement, which is necessary for outgrowth. Thus outgrowth is slowed, but not eliminated, in neurons from the myosin IIB KO mice.
Indexed as
Identifiers
11487639PMC6763133W1588249072What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.