ArticleBritish journal of pharmacology2001
2,5-Di-t-butyl-1,4-benzohydroquinone (BHQ) inhibits vascular L-type Ca(2+) channel via superoxide anion generation.
Article in British journal of pharmacology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 54 citations in OpenAlex.
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- Oxidative Regulation of Vascular CaAntioxidants (Basel, Switzerland) · 2022Review
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- Oxidative stress measured in vivo without an exogenous contrast agent using QUEST MRI.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2018Review
- Inhibition of Sarco-Endoplasmic Reticulum CaFrontiers in pharmacology · 2018Article
- MRI of rod cell compartment-specific function in disease and treatment in vivo.Progress in retinal and eye research · 2016Review
- Functional, electrophysiological and molecular docking analysis of the modulation of Cav 1.2 channels in rat vascular myocytes by murrayafoline A.British journal of pharmacology · 2016Article
- Playing with opening and closing of heterocycles: using the cusmano-ruccia reaction to develop a novel class of oxadiazolothiazinones, active as calcium channel modulators and P-glycoprotein inhibitors.Molecules (Basel, Switzerland) · 2014Article
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- The flavonoid scaffold as a template for the design of modulators of the vascular Ca(v) 1.2 channels.British journal of pharmacology · 2011Article
- (S)-reticuline induces vasorelaxation through the blockade of L-type Ca(2+) channels.Naunyn-Schmiedeberg's archives of pharmacology · 2009Article
- Interplay between SERCA and sarcolemmal Ca2+ efflux pathways controls spontaneous release of Ca2+ from the sarcoplasmic reticulum in rat ventricular myocytes.The Journal of physiology · 2004Article
- 3,5-Dibenzoyl-4-(3-phenoxyphenyl)-1,4-dihydro-2,6-dimethylpyridine (DP7) as a new multidrug resistance reverting agent devoid of effects on vascular smooth muscle contractility.British journal of pharmacology · 2004Article
- L-type Ca2+ channels activation and contraction elicited by myricetin on vascular smooth muscles.Naunyn-Schmiedeberg's archives of pharmacology · 2003Article
- Ca(2+) entry blocking and contractility promoting actions of norbormide in single rat caudal artery myocytes.British journal of pharmacology · 2002Article
- Quercetin as a novel activator of L-type Ca(2+) channels in rat tail artery smooth muscle cells.British journal of pharmacology · 2002Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of the present study was to investigate the effects of 2,5-di-t-butyl-1,4-benzohydroquinone (BHQ), an inhibitor of the sarco-endoplasmic reticulum Ca(2+)-ATPase (SERCA), on the whole-cell voltage-dependent L-type Ca(2+) current (I(Ca(L))) of freshly isolated smooth muscle cells from the rat tail artery using the patch-clamp technique. BHQ, added to the perfusion solution, reduced I(Ca(L)) in a concentration- (IC(50)=66.7 microM) and voltage-dependent manner. This inhibition was only partially reversible. BHQ shifted the voltage dependence of the steady-state inactivation curve to more negative potentials by 7 mV in the mid-potential of the curve, without affecting the activation curve as well as the time course of I(Ca(L)) inactivation. Preincubation of the cells either with 10 microM cyclopiazonic acid, a SERCA inhibitor, or with 3 mM diethyldithiocarbamate, an inhibitor of intracellular superoxide dismutase (SOD), did not modify BHQ inhibition of I(Ca(L)). On the contrary, this effect was no longer evident when SOD (250 u ml(-1)) was added to the perfusion medium. Either in the presence or in the absence of cells, BHQ gave rise to superoxide anion formation, which was markedly inhibited by the addition of SOD. These results indicate that, at micromolar concentrations, BHQ inhibits vascular I(Ca(L)) by giving rise to the formation of superoxide anion which in turn impairs the channel function.
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