ArticleBritish journal of pharmacology2001
High glucose induces cell death of cultured human aortic smooth muscle cells through the formation of hydrogen peroxide.
Article in British journal of pharmacology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 97 citations in OpenAlex.
- Hypotaurine Reduces Glucose-Mediated Vascular Calcification.Acta physiologica (Oxford, England) · 2025Article
- Integrative analysis of gene expression, protein abundance, and metabolomic profiling elucidates complex relationships in chronic hyperglycemia-induced changes in human aortic smooth muscle cells.Journal of biological engineering · 2024Article
- Analysis of Lemon Verbena Polyphenol Metabolome and Its Correlation with Oxidative Stress under Glucotoxic Conditions in Adipocyte.Journal of agricultural and food chemistry · 2024Article
- Sub-nanoliter metabolomics via mass spectrometry to characterize volume-limited samples.Nature communications · 2020Article
- Polyphenols Attenuate Highly-Glycosylated Haemoglobin-Induced Damage in Human Peritoneal Mesothelial Cells.Antioxidants (Basel, Switzerland) · 2020Article
- Advanced age protects resistance arteries of mouse skeletal muscle from oxidative stress through attenuating apoptosis induced by hydrogen peroxide.The Journal of physiology · 2019Article
- Effect of the PTHrP(1-34) analog abaloparatide on inducing chondrogenesis involves inhibition of intracellular reactive oxygen species production.Biochemical and biophysical research communications · 2019Article
- Parathyroid hormone-related protein exhibits antioxidant features in osteoblastic cells through its N-terminal and osteostatin domains.Bone & joint research · 2018Article
- Hyperglycemia enhances function and differentiation of adult rat cardiac fibroblasts.Canadian journal of physiology and pharmacology · 2014Article
- Differential regulation of human aortic smooth muscle cell proliferation by monocyte-derived macrophages from diabetic patients.PloS one · 2014Article
- Chronic supplementation of paeonol combined with danshensu for the improvement of vascular reactivity in the cerebral basilar artery of diabetic rats.International journal of molecular sciences · 2012Article
- Alterations in sarcoplasmic reticulum and mitochondrial functions in diabetic cardiomyopathy.Experimental and clinical cardiology · 2012Article
- Oxidative stress and heart failure in altered thyroid States.TheScientificWorldJournal · 2012Review
- Vascular endothelial growth factor protects post-ganglionic sympathetic neurones from the detrimental effects of hydrogen peroxide by increasing catalase.Acta physiologica (Oxford, England) · 2011Article
- Effects of the Cellcultured Acanthopanax senticosus Extract on Antioxidative Defense System and Membrane Fluidity in the Liver of Type 2 Diabetes Mouse.Journal of clinical biochemistry and nutrition · 2009Article
- Oxidative stress and COX cause hyper-responsiveness in vascular smooth muscle of the femoral artery from diabetic rats.British journal of pharmacology · 2008Article
- Contribution of aldose reductase to diabetic hyperproliferation of vascular smooth muscle cells.Diabetes · 2006Article
- Amadori adducts activate nuclear factor-kappaB-related proinflammatory genes in cultured human peritoneal mesothelial cells.British journal of pharmacology · 2005Article
- Catalase enzyme mutations and their association with diseases.Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology · 2004Review
- Glycosylated human oxyhaemoglobin activates nuclear factor-kappaB and activator protein-1 in cultured human aortic smooth muscle.British journal of pharmacology · 2003Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Alterations of the vessel structure, which is mainly determined by smooth muscle cells through cell growth and/or cell death mechanisms, are characteristic of diabetes complications. We analysed the influence of high glucose (22 mM) on cultured human aortic smooth muscle cell growth and death, as hyperglycaemia is considered one of the main factors involved in diabetic vasculopathy. Growth curves were performed over 96 h in medium containing 0.5% foetal calf serum. Cell number increased by 2 - 4 fold over the culture period in the presence of 5.5 mM (low) glucose, while a 20% reduction in final cell number was observed with high glucose. Under serum-free conditions, cell number remained constant in low glucose cultures, but a 40% decrease was observed in high glucose cultures, suggesting that high glucose may induce increased cell death rather than reduced proliferation. Reduced final cell number induced by high glucose was also observed after stimulation with 5 or 10% foetal calf serum. The possible participation of oxidative stress was investigated by co-incubating high glucose with different reactive oxygen species scavengers. Only catalase reversed the effect of high glucose. Intracellular H(2)O(2) content, visualized with 2',7'-dichlorofluorescein and quantified by flow cytometry, was increased after high glucose treatment. To investigate the cell death mechanism induced by high glucose, apoptosis and necrosis were quantified. No differences were observed regarding the apoptotic index between low and high glucose cultures, but lactate dehydrogenase activity was increased in high glucose cultures. In conclusion, high glucose promotes necrotic cell death through H(2)O(2) formation, which may participate in the development of diabetic vasculopathy.
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