ArticleThe Journal of molecular diagnostics : JMD2001
Laser-assisted microdissection of membrane-mounted paraffin sections for polymerase chain reaction analysis: identification of cell populations using immunohistochemistry and in situ hybridization.
Article in The Journal of molecular diagnostics : JMD, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 54 citations in OpenAlex.
- Myeloid antigen-presenting cell niches sustain antitumor T cells and license PD-1 blockade via CD28 costimulation.Cancer cell · 2021Article
- Isolation and gene expression profiling of intestinal epithelial cells: crypt isolation by calcium chelation from in vivo samples.Clinical and experimental gastroenterology · 2018Article
- Laser capture microdissection: Big data from small samples.Histology and histopathology · 2015Review
- Gut immune reconstitution in immune dysregulation, polyendocrinopathy, enteropathy, X-linked syndrome after hematopoietic stem cell transplantation.The Journal of allergy and clinical immunology · 2015Article
- Beyond laser microdissection technology: follow the yellow brick road for cancer research.American journal of cancer research · 2014Review
- FSH stimulates lipid biosynthesis in chicken adipose tissue by upregulating the expression of its receptor FSHR.Journal of lipid research · 2012Article
- Laser capture microdissection in forensic research: a review.International journal of legal medicine · 2010Review
- Immunoguided laser assisted microdissection techniques for DNA methylation analysis of archival tissue specimens.The Journal of molecular diagnostics : JMD · 2010Article
- No strong association between HER-2/neu protein overexpression and gene amplification in high-grade invasive urothelial carcinomas.Pathology oncology research : POR · 2008Article
- Laser capture microdissection in the genomic and proteomic era: targeting the genetic basis of cancer.International journal of clinical and experimental pathology · 2008Article
- Article
- Real-time quantitative PCR of microdissected paraffin-embedded breast carcinoma: an alternative method for HER-2/neu analysis.The Journal of molecular diagnostics : JMD · 2004Article
- Improved resolution by mounting of tissue sections for laser microdissection.Molecular pathology : MP · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Laser microbeam microdissection (LMM) is an increasingly important method for obtaining pure cell samples for genetic and proteomic analysis. Immunohistochemistry (IHC) and in situ hybridization (ISH) are useful techniques for targeting specific cell populations for microdissection but are difficult to apply with the tissue support membranes often used during LMM. Using detection of cytokeratins and Epstein-Barr virus gene products in head and neck carcinoma as a model, we describe optimized protocols for membrane and section preparation and for low temperature antigen retrieval that allow IHC and ISH to be used reliably on membrane mounted paraffin tissue sections. Visualization of cellular targets was markedly improved by staining and this could be further improved using a variety of optical media before microdissection. Tissue fragments thus stained were suitable for subsequent polymerase chain reaction analysis of extracted DNA using standard techniques. These IHC and ISH procedures are generally applicable and will be useful for detecting a wide range of antigens and nucleic acids in paraffin sections in conjunction with LMM.
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