ArticleMolecular and cellular biology2001
Partners of Rpb8p, a small subunit shared by yeast RNA polymerases I, II and III.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed.
- Molecular Evolution of the Archaeal DNA-Dependent RNA Polymerase: Cooperative Changes in Subunit Composition and Specific Domains of Small Subunits.International journal of molecular sciences · 2026Article
- Structure of the recombinant RNA polymerase from African Swine Fever Virus.Nature communications · 2024Article
- Never a dull enzyme, RNA polymerase II.Transcription · 2023Review
- Three human RNA polymerases interact with TFIIH via a common RPB6 subunit.Nucleic acids research · 2022Article
- Structural basis of RNA polymerase inhibition by viral and host factors.Nature communications · 2021Article
- Comparative CRISPR type III-based knockdown of essential genes in hyperthermophilicRNA biology · 2021Article
- Biogenesis of RNA Polymerases in Yeast.Frontiers in molecular biosciences · 2021Review
- Structure-Based Deep Mining Reveals First-Time Annotations for 46 Percent of the Dark Annotation Space of the 9,671-Member Superproteome of the Nucleocytoplasmic Large DNA Viruses.Journal of virology · 2020Article
- Article
- Evolution of multisubunit RNA polymerases in the three domains of life.Nature reviews. Microbiology · 2011Review
- Integrative structure modeling of macromolecular assemblies from proteomics data.Molecular & cellular proteomics : MCP · 2010Article
- A comparative analysis of DNA barcode microarray feature size.BMC genomics · 2009Article
- Budding yeast SSD1-V regulates transcript levels of many longevity genes and extends chronological life span in purified quiescent cells.Molecular biology of the cell · 2009Article
- Gene deletion and allelic replacement in the filamentous fungus Podospora anserina.Current genetics · 2008Article
- Orthologs of the small RPB8 subunit of the eukaryotic RNA polymerases are conserved in hyperthermophilic Crenarchaeota and "Korarchaeota".Biology direct · 2007Article
- Functional organization of the Rpb5 subunit shared by the three yeast RNA polymerases.Nucleic acids research · 2007Article
- Ancient origin, functional conservation and fast evolution of DNA-dependent RNA polymerase III.Nucleic acids research · 2006Article
- Lack of mitochondrial citrate synthase discloses a new meiotic checkpoint in a strict aerobe.The EMBO journal · 2002Article
- Hmo1, an HMG-box protein, belongs to the yeast ribosomal DNA transcription system.The EMBO journal · 2002Article
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8 authors.
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Abstract
Rpb8p, a subunit common to the three yeast RNA polymerases, is conserved among eukaryotes and absent from noneukaryotes. Defective mutants were found at an invariant GGLLM motif and at two other highly conserved amino acids. With one exception, they are clustered on the Rpb8p structure. They all impair a two-hybrid interaction with a fragment conserved in the largest subunits of RNA polymerases I (Rpa190p), II (Rpb1p), and III (Rpc160p). This fragment corresponds to the pore 1 module of the RNA polymerase II crystal structure and bears a highly conserved motif (P.I.KP.LW.GKQ) facing the GGLLM motif of Rpb8p. An RNA polymerase I mutant (rpa190-G728D) at the invariant glycyl of P.I.KP.LW.GKQ provokes a temperature-sensitive defect. Increasing the gene dosage of another common subunit, Rpb6p, suppresses this phenotype. It also suppresses a conditional growth defect observed when replacing Rpb8p by its human counterpart. Hence, Rpb6p and Rpb8p functionally interact in vivo. These two subunits are spatially separated by the pore 1 module and may also be possibly connected by the disorganized N half of Rpb6p, not included in the present structure data. Human Rpb6p is phosphorylated at its N-terminal Ser2, but an alanyl replacement at this position still complements an rpb6-Delta null allele. A two-hybrid interaction also occurs between Rpb8p and the product of orphan gene YGR089w. A ygr089-Delta null mutant has no detectable growth defect but aggravates the conditional growth defect of rpb8 mutants, suggesting that the interaction with Rpb8p may be physiologically relevant.
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