ArticleMolecular and cellular biology2001
Control of DNA rereplication via Cdc2 phosphorylation sites in the origin recognition complex.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
41 citing papers in PubMed, 90 citations in OpenAlex.
- Phosphorylation of Orc6 During Mitosis Regulates DNA Replication and Ribosome Biogenesis.Molecular and cellular biology · 2024Article
- Knockdown of Dinoflagellate CondensinMicroorganisms · 2020Article
- Mechanisms and regulation of DNA replication initiation in eukaryotes.Critical reviews in biochemistry and molecular biology · 2017Review
- Regulation of DNA replication proteins in parasitic protozoans: possible role of CDK-like kinases.Current genetics · 2016Review
- Plasma membrane/cell wall perturbation activates a novel cell cycle checkpoint during G1 in Saccharomyces cerevisiae.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Global analysis of protein expression and phosphorylation of three stages of Plasmodium falciparum intraerythrocytic development.Journal of proteome research · 2013Article
- Regulating DNA replication in eukarya.Cold Spring Harbor perspectives in biology · 2013Review
- Sequential steps in DNA replication are inhibited to ensure reduction of ploidy in meiosis.Molecular biology of the cell · 2013Article
- Genomic instability in cancer.Cold Spring Harbor perspectives in biology · 2013Review
- A quantitative model of the initiation of DNA replication in Saccharomyces cerevisiae predicts the effects of system perturbations.BMC systems biology · 2012Article
- Phosphorylation of ORC2 protein dissociates origin recognition complex from chromatin and replication origins.The Journal of biological chemistry · 2012Article
- Polo-like kinase 1 (Plk1): an Unexpected Player in DNA Replication.Cell division · 2012Article
- A yeast GSK-3 kinase Mck1 promotes Cdc6 degradation to inhibit DNA re-replication.PLoS genetics · 2012Article
- The dual role of autonomously replicating sequences as origins of replication and as silencers.Current genetics · 2009Review
- Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.PLoS biology · 2009Article
- Dynamic localization of the DNA replication proteins MCM5 and MCM7 in plants.Plant physiology · 2009Article
- Single particle EM studies of the Drosophila melanogaster origin recognition complex and evidence for DNA wrapping.Journal of structural biology · 2008Article
- Interplay between S-cyclin-dependent kinase and Dbf4-dependent kinase in controlling DNA replication through phosphorylation of yeast Mcm4 N-terminal domain.Molecular biology of the cell · 2008Article
- Once in a lifetime: strategies for preventing re-replication in prokaryotic and eukaryotic cells.EMBO reports · 2008Review
- Checkpoint effects and telomere amplification during DNA re-replication in fission yeast.BMC molecular biology · 2007Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Cdc2 kinase is a master regulator of cell cycle progression in the fission yeast Schizosaccharomyces pombe. Our data indicate that Cdc2 phosphorylates replication factor Orp2, a subunit of the origin recognition complex (ORC). Cdc2 phosphorylation of Orp2 appears to be one of multiple mechanisms by which Cdc2 prevents DNA rereplication in a single cell cycle. Cdc2 phosphorylation of Orp2 is not required for Cdc2 to activate DNA replication initiation. Phosphorylation of Orp2 appears first in S phase and becomes maximal in G(2) and M when Cdc2 kinase activity is required to prevent reinitiation of DNA replication. A mutant lacking Cdc2 phosphorylation sites in Orp2 (orp2-T4A) allowed greater rereplication of DNA than congenic orp2 wild-type strains when the limiting replication initiation factor Cdc18 was deregulated. Thus, Cdc2 phosphorylation of Orp2 may be redundant with regulation of Cdc18 for preventing reinitiation of DNA synthesis. Since Cdc2 phosphorylation sites are present in Orp2 (also known as Orc2) from yeasts to metazoans, we propose that cell cycle-regulated phosphorylation of the ORC provides a safety net to prevent DNA rereplication and resulting genetic instability.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.