ArticleThe Biochemical journal2001
Endoplasmic reticulum Ca2+-ATPase pump is up-regulated in calcium-transporting dental enamel cells: a non-housekeeping role for SERCA2b.
Article in The Biochemical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 34 citations in OpenAlex.
- Mitochondria modulate ameloblast CaFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022Article
- Structural and functional relations between the connective tissue and epithelium of enamel organ and their role during enamel maturation.Journal of molecular histology · 2021Article
- Calcium Transport in Specialized Dental Epithelia and Its Modulation by Fluoride.Frontiers in endocrinology · 2021Review
- Altered CaBiochimica et biophysica acta. Molecular cell research · 2018Review
- Evidence That Calcium Entry Into Calcium-Transporting Dental Enamel Cells Is Regulated by Cholecystokinin, Acetylcholine and ATP.Frontiers in physiology · 2018Article
- Enamel: Molecular identity of its transepithelial ion transport system.Cell calcium · 2017Review
- DENTAL ENAMEL FORMATION AND IMPLICATIONS FOR ORAL HEALTH AND DISEASE.Physiological reviews · 2017Review
- CaThe Journal of physiology · 2017Review
- Diseases caused by mutations in ORAI1 and STIM1.Annals of the New York Academy of Sciences · 2015Review
- Dental enamel cells express functional SOCE channels.Scientific reports · 2015Article
- Store-operated Ca2+ Entry Modulates the Expression of Enamel Genes.Journal of dental research · 2015Article
- Identification of novel candidate genes involved in mineralization of dental enamel by genome-wide transcript profiling.Journal of cellular physiology · 2012Article
- Expression of the sodium/calcium/potassium exchanger, NCKX4, in ameloblasts.Cells, tissues, organs · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dental enamel-forming cells face a major challenge to avoid the cytotoxic effects of excess calcium. We have characterized sarcoplasmic/endoplasmic reticulum calcium-ATPase pumps (SERCA) in rat enamel cells to address the proposal that non-mitochondrial calcium stores play a dominant role in transcellular calcium transport. A single major isoform, SERCA2b, was detected during the protein-secretory and calcium-transport stages of enamel formation using reverse-transcriptase PCR, cDNA cloning, Northern analysis and immunoblotting. Most importantly, SERCA2b exhibited a specific 3-fold up-regulation to high expression levels during calcium transport, as determined by quantitative immunoblotting and ATPase assays. Sensitivity of the calcium-dependent ATPase to thapsigargin and three other SERCA inhibitors was characterized. These findings indicate that enamel cells are well-equipped to sequester calcium in endoplasmic reticulum stores and so protect against calcium toxicity, associate SERCA with transcellular calcium transport for the first time, and establish SERCA2b as a molecular and pharmacological target for future investigations of calcium transcytosis. The observed physiological regulation in enamel cells contradicts the widespread perception that SERCA2b is restricted to general housekeeping duties.
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