ArticleThe Biochemical journal2001
Assembly of protein kinase CK2: investigation of complex formation between catalytic and regulatory subunits using a zinc-finger-deficient mutant of CK2beta.
Article in The Biochemical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 45 citations in OpenAlex.
- DNA damage response inhibition is an early event in cadmium-induced breast carcinogenesis.Toxicology and applied pharmacology · 2025Article
- Casein Kinase 2 Affects Epilepsy by Regulating Ion Channels: A Potential Mechanism.CNS & neurological disorders drug targets · 2024Review
- The regulatory subunits of CK2 complex mediate DNA damage response and virulence in Candida Glabrata.BMC microbiology · 2023Article
- Article
- Comparing Two Neurodevelopmental Disorders Linked to CK2: Okur-Chung Neurodevelopmental Syndrome and Poirier-Bienvenu Neurodevelopmental Syndrome-Two Sides of the Same Coin?Frontiers in molecular biosciences · 2022Review
- Splicing Interruption by Intron Variants inFrontiers in neuroscience · 2022Article
- Predictive functional, statistical and structural analysis ofFrontiers in molecular biosciences · 2022Article
- Clinical and genetic analysis of six Chinese children with Poirier-Bienvenu neurodevelopmental syndrome caused by CSNK2B mutation.Neurogenetics · 2021Article
- Disrupting the Constitutive, Homodimeric Protein-Protein Interface in CK2β Using a Biophysical Fragment-Based Approach.Journal of the American Chemical Society · 2016Article
- Identification of a novel protein interaction motif in the regulatory subunit of casein kinase 2.Molecular and cellular biology · 2014Article
- Involvement of Plasmodium falciparum protein kinase CK2 in the chromatin assembly pathway.BMC biology · 2012Article
- Regulation of DNA-dependent protein kinase by protein kinase CK2 in human glioblastoma cells.Oncogene · 2010Article
- Casein kinase 2 beta-subunit is a regulator of bone morphogenetic protein 2 signaling.Biophysical journal · 2010Article
- Differential regulation of mitogen- and stress-activated protein kinase-1 and -2 (MSK1 and MSK2) by CK2 following UV radiation.The Journal of biological chemistry · 2010Article
- CK2 is a novel negative regulator of NADPH oxidase and a neuroprotectant in mice after cerebral ischemia.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Structure-based design of small peptide inhibitors of protein kinase CK2 subunit interaction.The Biochemical journal · 2007Article
- B23 is a downstream target of polyamine-modulated CK2.Molecular and cellular biochemistry · 2005Article
- The multiple personalities of the regulatory subunit of protein kinase CK2: CK2 dependent and CK2 independent roles reveal a secret identity for CK2beta.International journal of biological sciences · 2005Article
- Live-cell fluorescence imaging reveals the dynamics of protein kinase CK2 individual subunits.Molecular and cellular biology · 2003Article
- Protein kinase CK2: structure, regulation and role in cellular decisions of life and death.The Biochemical journal · 2003Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein kinase CK2 is a tetrameric enzyme comprised of two regulatory subunits (CK2beta) and two catalytic subunits (CK2alpha and/or CK2alpha'). The crystal structure of dimeric CK2beta demonstrated that a zinc finger mediates CK2beta dimerization, therefore we constructed a mutant in which cysteine residues 109 and 114 were mutated to serine. Our objectives were to examine the effects of disrupting the zinc finger of the regulatory CK2beta subunit on CK2 tetramer assembly. Examination of this zinc-finger-deficient mutant of CK2beta using a yeast two-hybrid assay demonstrates that the mutant fails to form CK2beta homodimers. In order to extend these studies, we co-transfected COS-7 cells with epitope-tagged constructs and performed co-immunoprecipitation assays. The results from these studies demonstrate that the mutant fails to form CK2beta homodimers and fails to interact with catalytic CK2 subunits. Furthermore, we demonstrate that the mutant CK2beta is not appreciably phosphorylated in cells. Using in vitro binding assays, we demonstrated that the mutant CK2beta protein fails to interact with glutathione S-transferase-CK2alpha'. Finally, we demonstrate that the mutant is translated at an equivalent rate to wild-type CK2beta, but is degraded much more rapidly. Overall, our results are consistent with the model that beta-beta dimerization precedes incorporation of catalytic subunits into tetrameric CK2 complexes, and that beta-beta dimerization is a prerequisite for the stable incorporation of catalytic subunits into CK2 complexes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.