ArticleJournal of virology2001
Construction of an infectious cDNA clone of Aichi virus (a new member of the family Picornaviridae) and mutational analysis of a stem-loop structure at the 5' end of the genome.
Article in Journal of virology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 72 citations in OpenAlex.
- Isolation and molecular characteristics of D2 genotype of Aichivirus D in dairy cattle in China.Frontiers in veterinary science · 2025Article
- Article
- Advances and Breakthroughs in IRES-Directed Translation and Replication of Picornaviruses.mBio · 2023Review
- Article
- Recent advances in RNA structurome.Science China. Life sciences · 2022Review
- The 5'-end motif of Senecavirus A cDNA clone is genetically modified in 36 different ways for uncovering profiles of virus recovery.Frontiers in microbiology · 2022Article
- Animal and human RNA viruses: genetic variability and ability to overcome vaccines.Archives of microbiology · 2021Review
- Molecular characterization of the first reportedAccess microbiology · 2020Article
- Bacterial Stabilization of a Panel of Picornaviruses.mSphere · 2019Article
- Article
- The Roles of Picornavirus Untranslated Regions in Infection and Innate Immunity.Frontiers in microbiology · 2018Review
- Aichi virus 1: environmental occurrence and behavior.Pathogens (Basel, Switzerland) · 2015Review
- Widespread distribution and structural diversity of Type IV IRESs in members of Picornaviridae.Virology · 2015Article
- Picornavirus--host interactions to construct viral secretory membranes.Progress in molecular biology and translational science · 2015Review
- A complex comprising phosphatidylinositol 4-kinase IIIβ, ACBD3, and Aichi virus proteins enhances phosphatidylinositol 4-phosphate synthesis and is critical for formation of the viral replication complex.Journal of virology · 2014Article
- The 3A protein from multiple picornaviruses utilizes the golgi adaptor protein ACBD3 to recruit PI4KIIIβ.Journal of virology · 2012Article
- ACBD3-mediated recruitment of PI4KB to picornavirus RNA replication sites.The EMBO journal · 2012Article
- A distinct class of internal ribosomal entry site in members of the Kobuvirus and proposed Salivirus and Paraturdivirus genera of the Picornaviridae.Journal of virology · 2012Article
- Characterization of a canine homolog of human Aichivirus.Journal of virology · 2011Article
- Sequence analysis reveals mosaic genome of Aichi virus.Virology journal · 2011Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Aichi virus is the type species of a new genus, Kobuvirus, of the family Picornaviridae. In this study, we constructed a full-length cDNA clone of Aichi virus whose in vitro transcripts were infectious to Vero cells. During construction of the infectious cDNA clone, a novel sequence of 32 nucleotides was identified at the 5' end of the genome. Computer-assisted prediction of the secondary structure of the 5' end of the genome, including the novel sequence, suggested the formation of a stable stem-loop structure consisting of 42 nucleotides. The function of this stem-loop in virus replication was investigated using various site-directed mutants derived from the infectious cDNA clone. Our data indicated that correct folding of the stem-loop at the 5' end of the positive strand, but not at the 3' end of the negative strand, is critical for viral RNA replication. The primary sequence in the lower part of the stem was also suggested to be crucial for RNA replication. In contrast, nucleotide changes in the loop segment did not so severely reduce the efficiency of virus replication. A double mutant, in which both nucleotide stretches of the middle part of the stem were replaced by their complementary nucleotides, had efficient RNA replication and translation abilities but was unable to produce viruses. These results indicate that the stem-loop at the 5' end of the Aichi virus genome is an element involved in both viral RNA replication and production of infectious virus particles.
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