ArticleThe Journal of physiology2001
Direct measurement of single-channel Ca(2+) currents in bullfrog hair cells reveals two distinct channel subtypes.
Article in The Journal of physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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Who cites it
70 citing papers in PubMed, 113 citations in OpenAlex.
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- Multiple Calcium Channel Types with Unique Expression Patterns Mediate Retinal Signaling at Bipolar Cell Ribbon Synapses.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Article
- Cation Permeability of Voltage-Gated Hair Cell CaInternational journal of molecular sciences · 2022Article
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- Recent advances in cochlear hair cell nanophysiology: subcellular compartmentalization of electrical signaling in compact sensory cells.Faculty reviews · 2020Review
- The local translation ofAging · 2019Article
- Early functional alterations in membrane properties and neuronal degeneration are hallmarks of progressive hearing loss in NOD mice.Scientific reports · 2019Article
- Sodium-activated potassium channels shape peripheral auditory function and activity of the primary auditory neurons in mice.Scientific reports · 2019Article
- Otoprotective Effects of Stephania tetrandra S. Moore Herb Isolate against Acoustic Trauma.Journal of the Association for Research in Otolaryngology : JARO · 2018Article
- Voltage-Gated Calcium Channels: Key Players in Sensory Coding in the Retina and the Inner Ear.Physiological reviews · 2018Review
- Quantitative optical nanophysiology of CaNature communications · 2018Article
- Etiology of distinct membrane excitability in pre- and posthearing auditory neurons relies on activity of Cl- channel TMEM16A.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Early investigational drugs for hearing loss.Expert opinion on investigational drugs · 2015Review
- Hair cell-type dependent expression of basolateral ion channels shapes response dynamics in the frog utricle.Frontiers in cellular neuroscience · 2015Article
- Genetic, cellular, and functional evidence for Ca2+ inflow through Cav1.2 and Cav1.3 channels in murine spiral ganglion neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Fine Tuning of CaV1.3 Ca2+ channel properties in adult inner hair cells positioned in the most sensitive region of the Gerbil Cochlea.PloS one · 2014Article
- Prophylactic and therapeutic functions of drug combinations against noise-induced hearing loss.Hearing research · 2013Article
10 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
1. To confer their acute sensitivity to mechanical stimuli, hair cells employ Ca(2+) ions to mediate sharp electrical tuning and neurotransmitter release. We examined the diversity and properties of voltage-gated Ca(2+) channels in bullfrog saccular hair cells by means of perforated and cell-attached patch-clamp techniques. Whole-cell Ca(2+) current records provided hints that hair cells express L-type as well as dihydropyridine-insensitive Ca(2+) currents. 2. Single Ca(2+) channel records confirmed the presence of L-type channels, and a distinct Ca(2+) channel, which has sensitivity towards omega-conotoxin GVIA. Despite its sensitivity towards omega-conotoxin GVIA, the non-L-type channel cannot necessarily be considered as an N-type channel because of its distinct voltage-dependent gating properties. 3. Using 65 mM Ca(2+) as the charge carrier, the L-type channels were recruited at about -40 mV and showed a single-channel conductance of 13 pS. Under similar recording conditions, the non-L-type channels were activated at approximately -60 mV and had a single-channel conductance of approximately 16 pS. 4. The non-L-type channel exhibited at least two fast open time constants (tau(o) = 0.2 and 5 ms). In contrast, the L-type channels showed long openings (tau(o) = approximately 23 ms) that were enhanced by Bay K 8644, in addition to the brief openings (tau(o) = 0.3 and 10 ms). 5. The number of functional channels observed in patches of similar sizes suggests that Ca(2+) channels are expressed singly, in low-density clusters (2-15 channels) and in high-density clusters (20-80 channels). Co-localization of the two channel subtypes was observed in patches containing low-density clusters, but was rare in patches containing high-density clusters. 6. Finally, we confirmed the existence of two distinct Ca(2+) channel subtypes by using immunoblot and immunohistochemical techniques.
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