ArticleThe EMBO journal2001
Hda, a novel DnaA-related protein, regulates the replication cycle in Escherichia coli.
Article in The EMBO journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 147 papers.
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Who cites it
147 citing papers in PubMed, 271 citations in OpenAlex.
- Establishment of titration-based control of DNA replication inScience advances · 2026Article
- Article
- The loop domains of the E. coli β sliding clamp are required for Hda-dependent inactivation of DnaA.Genetics · 2026Article
- Phage Mu enlists the β-sliding clamp for late gene transcription.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- The Escherichia coli replication initiator DnaA is titrated on the chromosome.Nature communications · 2025Article
- Live tracking of replisomes reveals nutrient-dependent regulation of replication elongation rates in Caulobacter crescentus.Current biology : CB · 2025Article
- Real-time analysis of initiation regulation systems during the progression of the reconstituted chromosomal replication cycle.Scientific reports · 2025Article
- Negative DNA supercoiling enhances DARS2 binding of DNA-bending protein IHF in the activation of Fis-dependent ATP-DnaA production.Nucleic acids research · 2025Article
- Loss of SeqA confers low-level fluoroquinolone resistance through transcriptional reprogramming and RpoS dependence inAIMS microbiology · 2025Article
- Dispensability of extrinsic DnaA regulators inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Direct single-cell observation of a keyScience advances · 2024Article
- Mapping theMicrobiology (Reading, England) · 2024Article
- LPS-DephosphorylatingMicroorganisms · 2024Article
- Open Questions about the Roles of DnaA, Related Proteins, and Hyperstructure Dynamics in the Cell Cycle.Life (Basel, Switzerland) · 2023Review
- IHF and Fis asInternational journal of molecular sciences · 2023Review
- Regulatory elements coordinating initiation of chromosome replication to theProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Rapid profiling of DNA replication dynamics using mass spectrometry-based analysis of nascent DNA.The Journal of cell biology · 2023Article
- Borrelia burgdorferi DnaA and the Nucleoid-Associated Protein EbfC Coordinate Expression of theJournal of bacteriology · 2023Article
- Mutations identified in engineeredFrontiers in microbiology · 2023Article
- Robust replication initiation from coupled homeostatic mechanisms.Nature communications · 2022Article
87 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The bacterial DnaA protein binds to the chromosomal origin of replication to trigger a series of initiation reactions, which leads to the loading of DNA polymerase III. In Escherichia coli, once this polymerase initiates DNA synthesis, ATP bound to DnaA is efficiently hydrolyzed to yield the ADP-bound inactivated form. This negative regulation of DnaA, which occurs through interaction with the beta-subunit sliding clamp configuration of the polymerase, functions in the temporal blocking of re-initiation. Here we show that the novel DnaA-related protein, Hda, from E.coli is essential for this regulatory inactivation of DnaA in vitro and in vivo. Our results indicate that the hda gene is required to prevent over-initiation of chromosomal replication and for cell viability. Hda belongs to the chaperone-like ATPase family, AAA(+), as do DnaA and certain eukaryotic proteins essential for the initiation of DNA replication. We propose that the once-per-cell-cycle rule of replication depends on the timely interaction of AAA(+) proteins that comprise the apparatus regulating the activity of the initiator of replication.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.