ArticleProceedings of the National Academy of Sciences of the United States of America2001
A mutant plasma membrane ATPase, Pma1-10, is defective in stability at the yeast cell surface.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed, 76 citations in OpenAlex.
- Cyclo(Pro-Tyr) elicits conserved cellular damage in fungi by targeting the [HCommunications biology · 2024Article
- Developing novel antifungals: lessons from G protein-coupled receptors.Trends in pharmacological sciences · 2023Review
- Caveolin-1 stabilizes ATP7A, a copper transporter for extracellular SOD, in vascular tissue to maintain endothelial function.American journal of physiology. Cell physiology · 2020Article
- Sphingolipid Effects on the Plasma Membrane Produced by Addition of Fumonisin B1 to Maize Embryos.Plants (Basel, Switzerland) · 2020Article
- The Oligomeric State of the Plasma Membrane H⁺-ATPase fromMolecules (Basel, Switzerland) · 2019Article
- The Essential Neo1 Protein from Budding Yeast Plays a Role in Establishing Aminophospholipid Asymmetry of the Plasma Membrane.The Journal of biological chemistry · 2016Article
- Raft-like membrane domains in pathogenic microorganisms.Current topics in membranes · 2015Review
- Loss of vacuolar H+-ATPase activity in organelles signals ubiquitination and endocytosis of the yeast plasma membrane proton pump Pma1p.The Journal of biological chemistry · 2014Article
- Inositol phosphosphingolipid phospholipase C1 regulates plasma membrane ATPase (Pma1) stability in Cryptococcus neoformans.FEBS letters · 2014Article
- Protein homeostasis at the plasma membrane.Physiology (Bethesda, Md.) · 2014Review
- Endocytic regulation of alkali metal transport proteins in mammals, yeast and plants.Current genetics · 2013Review
- Rapid response of the yeast plasma membrane proteome to salt stress.Molecular & cellular proteomics : MCP · 2011Article
- Routing misfolded proteins through the multivesicular body (MVB) pathway protects against proteotoxicity.The Journal of biological chemistry · 2011Article
- Protein quality control at the plasma membrane.Current opinion in cell biology · 2011Review
- pH-dependent cargo sorting from the Golgi.The Journal of biological chemistry · 2011Article
- Quality control for unfolded proteins at the plasma membrane.The Journal of cell biology · 2010Article
- Homoserine toxicity in Saccharomyces cerevisiae and Candida albicans homoserine kinase (thr1Delta) mutants.Eukaryotic cell · 2010Article
- Membrane rafts are involved in intracellular miconazole accumulation in yeast cells.The Journal of biological chemistry · 2009Article
- A mutant plasma membrane protein is stabilized upon loss of Yvh1, a novel ribosome assembly factor.Genetics · 2009Article
- Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Pma1 is a plasma membrane H(+)-ATPase whose activity at the cell surface is essential for cell viability. In this paper we describe a temperature-sensitive pma1 allele, pma1-10 (with two point mutations in the first cytoplasmic loop of Pma1), in which the newly synthesized mutant protein fails to remain stable at the cell surface at 37 degrees C. Instead, Pma1-10 appears to undergo internalization for vacuolar degradation in a manner dependent on End4, Vps27, Doa4, and Pep4. By contrast with wild-type Pma1, mutant Pma1-10 is hypophosphorylated and fails to associate with a Triton-insoluble fraction at 37 degrees C, suggesting failure to enter lipid rafts. Kinetic analysis reveals that, at the permissive temperature, newly synthesized Pma1-10 acquires Triton-insolubility before becoming stabilized. We suggest that phosphorylation and lipid raft association may play important roles in maintaining protein stability at the plasma membrane.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.