ArticleProceedings of the National Academy of Sciences of the United States of America2001
Polar targeting of Shigella virulence factor IcsA in Enterobacteriacae and Vibrio.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed.
- A broad spectrum Shigella vaccine based on VirGNPJ vaccines · 2025Article
- Shigella virulence protein VirG is a broadly protective antigen and vaccine candidate.NPJ vaccines · 2024Article
- Physical properties of the bacterial outer membrane.Nature reviews. Microbiology · 2022Review
- Mechanistic insight into bacterial entrapment by septin cage reconstitution.Nature communications · 2021Article
- Direct binding of polymeric GBP1 to LPS disrupts bacterial cell envelope functions.The EMBO journal · 2020Article
- Review
- Taming the Triskelion: Bacterial Manipulation of Clathrin.Microbiology and molecular biology reviews : MMBR · 2019Review
- Article
- Protein polarization driven by nucleoid exclusion of DnaK(HSP70)-substrate complexes.Nature communications · 2018Article
- Article
- Super-Resolution Imaging of Protein Secretion Systems and the Cell Surface of Gram-Negative Bacteria.Frontiers in cellular and infection microbiology · 2017Review
- Molecular and Cellular Mechanisms of Shigella flexneri Dissemination.Frontiers in cellular and infection microbiology · 2016Review
- Protein-protein interactions and the spatiotemporal dynamics of bacterial outer membrane proteins.Current opinion in structural biology · 2015Review
- Multi-color imaging of the bacterial nucleoid and division proteins with blue, orange, and near-infrared fluorescent proteins.Frontiers in microbiology · 2015Article
- Delineation of polar localization domains of Agrobacterium tumefaciens type IV secretion apparatus proteins VirB4 and VirB11.MicrobiologyOpen · 2014Article
- Polar localization of Escherichia coli chemoreceptors requires an intact Tol-Pal complex.Molecular microbiology · 2014Article
- PBP deletion mutants of Escherichia coli exhibit irregular distribution of MreB at the deformed zones.Current microbiology · 2014Article
- Biogenesis of YidC cytoplasmic membrane substrates is required for positioning of autotransporter IcsA at future poles.Journal of bacteriology · 2014Article
- Surface display of the HPV L1 capsid protein by the autotransporter Shigella IcsA.Journal of microbiology (Seoul, Korea) · 2014Article
- How do bacteria localize proteins to the cell pole?Journal of cell science · 2014Review
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Authors and funding
4 authors.
Funding
Abstract
Asymmetric localization is key to the proper function of certain prokaryotic proteins important to virulence, chemotaxis, cell division, development, motility, and adhesion. Shigella IcsA is localized to the old pole of the bacterium, where it mediates assembly of an actin tail inside infected mammalian cells. IcsA (VirG) is essential to Shigella intracellular motility and virulence. We used translational fusions between portions of IcsA and the green fluorescent protein (GFP) to determine the regions of IcsA that are necessary and sufficient for its targeting to the bacterial old pole. An IcsA-GFP fusion that lacks a signal peptide localized to the old pole, indicating that signal peptide-mediated secretion is not required for polar localization. Two regions within IcsA were required for localization of an IcsA-GFP fusion to the old pole. Further characterization of these regions indicated that amino acids 1-104 and 507-620 were each independently sufficient for polar localization. Finally, when expressed in Escherichia coli, Salmonella typhimurium, Yersinia pseudotuberculosis, and Vibrio cholerae, each of the two targeting regions localized to the pole, indicating that the mechanism of polar targeting used by IcsA is present generally among Enterobacteriacae and Vibrio.
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