ArticleThe Journal of general physiology2001
A kinetic analysis of calcium-triggered exocytosis.
Article in The Journal of general physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 25 citations in OpenAlex.
- miRNA-132-5p mediates a negative feedback regulation of IL-8 secretion through S100A8/A9 downregulation in neutrophil-like HL-60 cells.Frontiers in immunology · 2023Article
- Unraveling the mechanisms of calcium-dependent secretion.The Journal of general physiology · 2019Review
- Differential Release of Exocytosis Marker Dyes Indicates Stimulation-Dependent Regulation of Synaptic Activity.Frontiers in neuroscience · 2019Article
- Lipid-dependence of target membrane stability during influenza viral fusion.Journal of cell science · 2018Article
- Quantification of exocytosis kinetics by DIC image analysis of cortical lawns.Journal of chemical biology · 2014Article
- Dissecting the regulation of pollen tube growth by modeling the interplay of hydrodynamics, cell wall and ion dynamics.Frontiers in plant science · 2014Article
- Spatiotemporal resolution of mast cell granule exocytosis reveals correlation with Ca2+ wave initiation.Journal of cell science · 2012Article
- Regulator or driving force? The role of turgor pressure in oscillatory plant cell growth.PloS one · 2011Article
- Specific lipids supply critical negative spontaneous curvature--an essential component of native Ca2+-triggered membrane fusion.Biophysical journal · 2008Article
- Comparative analysis of tandem C2 domains from the mammalian synaptotagmin family.The Biochemical journal · 2004Article
- Kinetically differentiating influenza hemagglutinin fusion and hemifusion machines.Biophysical journal · 2003Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
Although the relationship between exocytosis and calcium is fundamental both to synaptic and nonneuronal secretory function, analysis is problematic because of the temporal and spatial properties of calcium, and the fact that vesicle transport, priming, retrieval, and recycling are coupled. By analyzing the kinetics of sea urchin egg secretory vesicle exocytosis in vitro, the final steps of exocytosis are resolved. These steps are modeled as a three-state system: activated, committed, and fused, where interstate transitions are given by the probabilities that an active fusion complex commits (alpha) and that a committed fusion complex results in fusion, p. The number of committed complexes per vesicle docking site is Poisson distributed with mean n. Experimentally, p and n increase with increasing calcium, whereas alpha and the pn ratio remain constant, reducing the kinetic description to only one calcium-dependent, controlling variable, n. On average, the calcium dependence of the maximum rate (R(max)) and the time to reach R(max) (T(peak)) are described by the calcium dependence of n. Thus, the nonlinear relationship between the free calcium concentration and the rate of exocytosis can be explained solely by the calcium dependence of the distribution of fusion complexes at vesicle docking sites.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.