ArticleThe Journal of general physiology2001
The relationship between cAMP, Ca(2)+, and transport of CFTR to the plasma membrane.
Article in The Journal of general physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 29 citations in OpenAlex.
- Role of ion channels in the functional response of conjunctival goblet cells to dry eye.American journal of physiology. Cell physiology · 2018Article
- Molecular motors and apical CFTR traffic in epithelia.International journal of molecular sciences · 2013Review
- Differential effects of lysophospholipids on exocytosis in rat PC12 cells.Journal of neural transmission (Vienna, Austria : 1996) · 2010Article
- PKC epsilon facilitates recovery of exocytosis after an exhausting stimulation.Pflugers Archiv : European journal of physiology · 2009Article
- Roles of cholesterol in vesicle fusion and motion.Biophysical journal · 2009Article
- Differential effects of ceramide species on exocytosis in rat PC12 cells.Experimental brain research · 2007Article
- Twenty odd years of stretch-sensitive channels.Pflugers Archiv : European journal of physiology · 2006Review
- A cyclic GMP-dependent calcium-activated chloride current in smooth-muscle cells from rat mesenteric resistance arteries.The Journal of general physiology · 2004Article
- CFTR chloride channels are regulated by a SNAP-23/syntaxin 1A complex.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- The number of K(+) channels in the plasma membrane of guard cell protoplasts changes in parallel with the surface area.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- CFTR: covalent modification of cysteine-substituted channels expressed in Xenopus oocytes shows that activation is due to the opening of channels resident in the plasma membrane.The Journal of general physiology · 2001Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The mechanism whereby cAMP stimulates Cl(-) flux through CFTR ion channels in secretory epithelia remains controversial. It is generally accepted that phosphorylation by cAMP-dependent protein kinase increases the open probability of the CFTR channel. A more controversial hypothesis is that cAMP triggers the translocation of CFTR from an intracellular pool to the cell surface. We have monitored membrane turnover in Calu-3 cells, a cell line derived from human airway submucosal glands that expresses high levels of CFTR using membrane capacitance and FM1-43 fluorescence measurements. Using a conventional capacitance measurement technique, we observe an apparent increase in membrane capacitance in most cells that exhibit an increase in Cl(-) current. However, after we carefully correct our recordings for changes in membrane conductance, the apparent changes in capacitance are eliminated. Measurements using the fluorescent membrane marker FM1-43 also indicate that no changes in membrane turnover accompany the activation of CFTR. Robust membrane insertion can be triggered with photorelease of caged Ca(2)+ in Calu-3 cells. However, no increase in Cl(-) current accompanies Ca(2)+-evoked membrane fusion. We conclude that neither increases in cAMP or Ca(2)+ lead to transport of CFTR to the plasma membrane in Calu-3 cells. In addition, we conclude that membrane capacitance measurements must be interpreted with caution when large changes in membrane conductance occur.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.