ArticleJournal of clinical microbiology2001
Detection and quantification of infectious hypodermal and hematopoietic necrosis virus and white spot virus in shrimp using real-time quantitative PCR and SYBR Green chemistry.
Article in Journal of clinical microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 171 citations in OpenAlex.
- Shrimp Aquaculture Under Viral Threats: Insights into Pathogens and Control Strategies.Food and environmental virology · 2026Review
- Class B scavenger receptor resists WSSV replication by recognizing the viral lipid molecule and promoting phagocytosis.Journal of virology · 2025Article
- Parvoviruses of Aquatic Animals.Pathogens (Basel, Switzerland) · 2024Review
- Experimental Infection Models and Their Usefulness for White Spot Syndrome Virus (WSSV) Research in Shrimp.Viruses · 2024Review
- Coinfection with Yellow Head Virus Genotype 8 (YHV-8) and Oriental Wenrivirus 1 (OWV1) in WildViruses · 2023Article
- Article
- Diversity of single-stranded DNA containing viruses in shrimp.Virusdisease · 2019Review
- Multiplex SYBR Green and duplex TaqMan real-time PCR assays for the detection of Photorhabdus Insect-Related (Pir) toxin genes pirA and pirB.Molecular and cellular probes · 2019Article
- Quantification of M13 and T7 bacteriophages by TaqMan and SYBR green qPCR.Journal of virological methods · 2018Article
- Scavenger Receptor C Mediates Phagocytosis of White Spot Syndrome Virus and Restricts Virus Proliferation in Shrimp.PLoS pathogens · 2016Article
- Development of duplex real-time PCR for the detection of WSSV and PstDV1 in cultivated shrimp.BMC veterinary research · 2014Article
- Association of dual viral infection with mortality of Pacific white shrimp (Litopenaeus vannamei) in culture ponds in India.Virusdisease · 2014Article
- Genomics, Molecular Epidemiology and Diagnostics of Infectious hypodermal and hematopoietic necrosis virus.Indian journal of virology : an official organ of Indian Virological Society · 2012Article
- Biology, Host Range, Pathogenesis and Diagnosis of White spot syndrome virus.Indian journal of virology : an official organ of Indian Virological Society · 2012Article
- Enzyme E2 from Chinese white shrimp inhibits replication of white spot syndrome virus and ubiquitinates its RING domain proteins.Journal of virology · 2011Article
- Virus diseases of farmed shrimp in the Western Hemisphere (the Americas): a review.Journal of invertebrate pathology · 2011Review
- Molecular cloning and characterization of the translationally controlled tumor protein from Fenneropenaeus chinensis.Molecular biology reports · 2009Article
- Overproduction of exopolysaccharides by an Escherichia coli K-12 rpoS mutant in response to osmotic stress.Applied and environmental microbiology · 2009Article
- Reduced PCR sensitivity due to impaired DNA recovery with the MagNA Pure LC total nucleic acid isolation kit.Journal of clinical microbiology · 2005Article
- Isolation and characterization of a TERMINAL FLOWER homolog and its correlation with juvenility in citrus.Plant physiology · 2004Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
A rapid and highly sensitive real-time PCR detection and quantification method for infectious hypodermal and hematopoietic necrosis virus (IHHNV), a single-stranded DNA virus, and white spot virus (WSV), a double-stranded DNA (dsDNA) virus infecting penaeid shrimp (Penaeus sp.), was developed using the GeneAmp 5700 sequence detection system coupled with SYBR Green chemistry. The PCR mixture contains a fluorescence dye, SYBR Green, which upon binding to dsDNA exhibits fluorescence enhancement. The enhancement of fluorescence was proportional to the initial concentration of the template DNA. A linear relationship was observed between the amount of input plasmid DNA and cycle threshold (C(T)) values over a range of 1 to 10(5) copies of the viral genome. To control the variation in sampling and processing among samples, the shrimp beta-actin gene was amplified in parallel with the viral DNA. The C(T) values of IHHNV- and WSV-infected samples were used to determine absolute viral copy numbers from the standard C(T) curves of these viruses. For each virus and its beta-actin control, the specificity of amplification was monitored by using the dissociation curve of the amplified product. Using genomic DNA as a template, SYBR Green PCR was found to be 100- to 2000-fold more sensitive than conventional PCR, depending on the virus, for the samples tested. The results demonstrate that SYBR Green PCR can be used as a rapid and highly sensitive detection and quantification method for shrimp viruses and that it is amenable to high-throughout assay.
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