ArticleJournal of clinical microbiology2001
Identification of 54 mycobacterial species by PCR-restriction fragment length polymorphism analysis of the hsp65 gene.
Article in Journal of clinical microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed.
- Trial
- Rapid and accurate identification and differentiation ofFrontiers in public health · 2024Article
- Mycobacterial Infections in Invasive Turtle Species in Poland.Pathogens (Basel, Switzerland) · 2023Article
- The Re-Identification of Previously Unidentifiable Clinical Non-Tuberculous Mycobacterial Isolates Shows Great Species Diversity and the Presence of Other Acid-Fast Genera.Pathogens (Basel, Switzerland) · 2022Article
- Identification and Characterisation of Nontuberculous Mycobacteria in African Buffaloes (Microorganisms · 2022Article
- Molecular characterization ofIranian journal of veterinary research · 2022Article
- Characterization of Clinical Isolates of Mycobacterium simiae Using Drug Susceptibility Tests and Molecular Analyses.Current microbiology · 2021Article
- Identification of mycobacteria species by molecular methods.International wound journal · 2020Article
- Evaluation of a membrane hybridization array for detection of Mycobacterium tuberculosis complex and resistance to isoniazid and rifampin in sputum specimens, mycobacterial liquid cultures, and clinical isolates.The Kaohsiung journal of medical sciences · 2019Article
- Nontuberculous Mycobacteria Persistence in a Cell Model Mimicking Alveolar Macrophages.Microorganisms · 2019Article
- A New Single Gene Differential Biomarker forFrontiers in microbiology · 2019Article
- Microbial Identification Using DNA Target Amplification and Sequencing: Clinical Utility and Impact on Patient Management.Open forum infectious diseases · 2018Review
- Nontuberculous mycobacterial lung disease in a high tuberculosis incidence setting in Brazil.Jornal brasileiro de pneumologia : publicacao oficial da Sociedade Brasileira de Pneumologia e Tisilogia · 2018Article
- Mycobacteriosis and Tuberculosis: Laboratory Diagnosis.The open microbiology journal · 2018Review
- Rapid identification of mycobacteria from positive MGIT broths of primary cultures by MALDI-TOF mass spectrometry.PloS one · 2018Article
- Early detection of Mycobacterium tuberculosis complex in BACTEC MGIT cultures using nucleic acid amplification.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2016Article
- Identification of Species of Nontuberculous Mycobacteria Clinical Isolates from 8 Provinces of China.BioMed research international · 2016Article
- Epidemiology of nontuberculous mycobacteria among patients with cystic fibrosis in Scandinavia.Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society · 2015Observational
- Occurrence of Mycobacterium bovis and non-tuberculous mycobacteria (NTM) in raw and pasteurized milk in the northwestern region of Paraná, Brazil.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2014Article
- Isolation of Mycobacterium branderi, an unusual species from an acute myelogenous leukemia patient.Avicenna journal of medicine · 2014Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A total of 121 reference and clinical strains of both slowly and rapidly growing mycobacteria belonging to 54 species were studied for restriction fragment length polymorphism of a PCR-amplified 439-bp segment of the gene encoding the 65-kDa heat shock protein. Restriction digests were separated by 10% polyacrylamide gel electrophoresis (PAGE). By including a size standard in each sample, the restriction fragment profile was calculated using a computer-aided comparison program. An algorithm describing these 54 species (including 22 species not previously described) is proposed. We found that this assay based on 10% PAGE provided a more precise estimate than that based on agarose gel electrophoresis of the real size of restriction fragments as deduced from the sequence analysis and allowed identification of mycobacteria whose PCR-restriction fragment length polymorphism analysis patterns were unequivocally identified by fragments shorter than 60 bp.
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