ArticleApplied and environmental microbiology2001
Utilization of the rpoB gene as a specific chromosomal marker for real-time PCR detection of Bacillus anthracis.
Article in Applied and environmental microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers, 1 of them a synthesis that pooled it.
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Who cites it
52 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Implications of limits of detection of various methods for Bacillus anthracis in computing risks to human health.Applied and environmental microbiology · 2009Pooled it
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- The Use of Xenosurveillance to Detect Human Bacteria, Parasites, and Viruses in Mosquito Bloodmeals.The American journal of tropical medicine and hygiene · 2017Article
- Identification and validation of specific markers of Bacillus anthracis spores by proteomics and genomics approaches.Molecular & cellular proteomics : MCP · 2014Article
- In silico and in vitro evaluation of PCR-based assays for the detection of Bacillus anthracis chromosomal signature sequences.Virulence · 2013Article
- Phylogenetic diversity of the Bacillus pumilus group and the marine ecotype revealed by multilocus sequence analysis.PloS one · 2013Article
- Existence of separate domains in lysin PlyG for recognizing Bacillus anthracis spores and vegetative cells.Antimicrobial agents and chemotherapy · 2012Article
- Real-time polymerase chain reaction assay for rapid and sensitive detection of anthrax spores in spiked soil and talcum powder.Folia microbiologica · 2012Article
- Rugged single domain antibody detection elements for Bacillus anthracis spores and vegetative cells.PloS one · 2012Article
- Positions of Trp codons in the leader peptide-coding region of the at operon influence anti-trap synthesis and trp operon expression in Bacillus licheniformis.Journal of bacteriology · 2010Article
- Detection of B. anthracis spores and vegetative cells with the same monoclonal antibodies.PloS one · 2009Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The potential use of Bacillus anthracis as a weapon of mass destruction poses a threat to humans, domesticated animals, and wildlife and necessitates the need for a rapid and highly specific detection assay. We have developed a real-time PCR-based assay for the specific detection of B. anthracis by taking advantage of the unique nucleotide sequence of the B. anthracis rpoB gene. Variable region 1 of the rpoB gene was sequenced from 36 Bacillus strains, including 16 B. anthracis strains and 20 other related bacilli, and four nucleotides specific for B. anthracis were identified. PCR primers were selected so that two B. anthracis-specific nucleotides were at their 3' ends, whereas the remaining bases were specific to the probe region. This format permitted the PCR reactions to be performed on a LightCycler via fluorescence resonance energy transfer (FRET). The assay was found to be specific for 144 B. anthracis strains from different geographical locations and did not cross-react with other related bacilli (175 strains), with the exception of one strain. The PCR assay can be performed on isolated DNA as well as crude vegetative cell lysates in less than 1 h. Therefore, the rpoB-FRET assay could be used as a new chromosomal marker for rapid detection of B. anthracis.
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