ArticleApplied and environmental microbiology2001
Ecological aspects of ntcA gene expression and its use as an indicator of the nitrogen status of marine Synechococcus spp.
Article in Applied and environmental microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed, 109 citations in OpenAlex.
- Article
- Nutrient-driven regulation of saxitoxin gene expression and toxin production in Raphidiopsis raciborskii (Cyanobacteria).Journal of phycology · 2025Article
- MarineMicrobiology spectrum · 2022Article
- Understanding the Variation of Bacteria in Response to Summertime Oxygen Depletion in Water Column of Bohai Sea.Frontiers in microbiology · 2022Article
- Heterocyst Development and Diazotrophic Growth ofLife (Basel, Switzerland) · 2020Article
- Phytoplankton transcriptomic and physiological responses to fixed nitrogen in the California current system.PloS one · 2020Article
- Metabolomic Fingerprints of Individual Algal Cells Using the Single-Probe Mass Spectrometry Technique.Frontiers in plant science · 2018Article
- Article
- Glucose Uptake inFrontiers in microbiology · 2017Article
- mdRNA-Seq analysis of marine microbial communities from the northern Red Sea.Scientific reports · 2016Article
- Bridging the gap between omics and earth system science to better understand how environmental change impacts marine microbes.Global change biology · 2016Review
- Physiological Studies of Glutamine Synthetases I and III from Synechococcus sp. WH7803 Reveal Differential Regulation.Frontiers in microbiology · 2016Article
- Genetic Diversity Affects the Daily Transcriptional Oscillations of Marine Microbial Populations.PloS one · 2016Article
- Long-term monitoring reveals carbon-nitrogen metabolism key to microcystin production in eutrophic lakes.Frontiers in microbiology · 2015Article
- Microcystin production and regulation under nutrient stress conditions in toxic microcystis strains.Applied and environmental microbiology · 2014Article
- Microspatial gene expression patterns in the Amazon River Plume.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- A microarray for assessing transcription from pelagic marine microbial taxa.The ISME journal · 2014Article
- Impact of DNA damaging agents on genome-wide transcriptional profiles in two marine Synechococcus species.Frontiers in microbiology · 2013Article
- Decoupling of ammonium regulation and ntcA transcription in the diazotrophic marine cyanobacterium Trichodesmium sp. IMS101.The ISME journal · 2012Article
- Diversity and Distribution of Marine Synechococcus: Multiple Gene Phylogenies for Consensus Classification and Development of qPCR Assays for Sensitive Measurement of Clades in the Ocean.Frontiers in microbiology · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nitrogen nutrition in cyanobacteria is regulated by NtcA, a transcriptional activator that is subject to negative control by ammonium. Using Synechococcus sp. strain WH7803 as a model organism, we show that ntcA expression was induced when cells were exposed to nitrogen stress but not when they were subjected to phosphorus or iron deprivation. Transcript levels accumulated in cells grown on a variety of inorganic and organic nitrogen sources, with the sole exception of ammonium. ntcA transcription was induced when ammonium levels dropped below 1 microM and reached maximal levels within 2 h. Furthermore, the addition of more than 1 microM ammonium led to a rapid decline in ntcA mRNA. The negative effect of ammonium was prevented by the addition of L-methionine-D,L-sulfoximine (MSX) and azaserine, inhibitors of ammonium assimilation. Thus, basal ntcA transcript levels are indicative of ammonium utilization. Conversely, the highest ntcA transcript levels were found in cells lacking a nitrogen source capable of supporting growth. Therefore, maximal ntcA expression would indicate nitrogen deprivation. This state of nitrogen deprivation was induced by a 1-h incubation with MSX. The rapid response of ntcA gene expression to the addition of ammonium and MSX was used to design a protocol for assessing relative ntcA transcript levels in field populations of cyanobacteria, from which their nitrogen status can be inferred. ntcA was basally expressed in Synechococcus at a nutrient-enriched site at the northern tip of the Gulf of Aqaba, Red Sea. Therefore, these cyanobacteria were not nitrogen stressed, and their nitrogen requirements were met by regenerated nitrogen in the form of ammonium.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.