ArticleClinical and experimental immunology2001
Characterization of conditions for the primary culture of human small intestinal epithelial cells.
Article in Clinical and experimental immunology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 30 citations in OpenAlex.
- Integrating Human Intestinal Organoids into FDA's New Approach Methodologies for Drug Discovery.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Culture media and format alter cellular composition and barrier integrity of porcine colonoid-derived monolayers.Tissue barriers · 2024Article
- Gut-on-a-chip models for dissecting the gut microbiology and physiology.APL bioengineering · 2023Review
- Avian Cell Culture Models to Study Immunomodulatory Properties of Bioactive Products.Animals : an open access journal from MDPI · 2022Review
- Toward Xeno-Free Differentiation of Human Induced Pluripotent Stem Cell-Derived Small Intestinal Epithelial Cells.International journal of molecular sciences · 2022Article
- Cytochrome P450 expression, induction and activity in human induced pluripotent stem cell-derived intestinal organoids and comparison with primary human intestinal epithelial cells and Caco-2 cells.Archives of toxicology · 2021Article
- Particulate Matter Decreases Intestinal Barrier-Associated Proteins Levels in 3D Human Intestinal Model.International journal of environmental research and public health · 2020Article
- Spheres of Influence: Insights intoMicroorganisms · 2020Review
- Self-organized intestinal epithelial monolayers in crypt and villus-like domains show effective barrier function.Scientific reports · 2019Article
- Advances and Current Challenges in IntestinalFrontiers in bioengineering and biotechnology · 2019Review
- Alternative functional in vitro models of human intestinal epithelia.Frontiers in pharmacology · 2013Article
- Endocrine differentiation of rat enterocytes in long-term three-dimensional co-culture with intestinal myofibroblasts.In vitro cellular & developmental biology. Animal · 2011Article
- Role of intestinal epithelial cells in immune effects mediated by gram-positive probiotic bacteria: involvement of toll-like receptors.Clinical and diagnostic laboratory immunology · 2005Article
- Establishment and characterization of a primary canine duodenal epithelial cell culture.In vitro cellular & developmental biology. AnimalArticle
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Intestinal epithelial cells (IECs) are important for many aspects of gut physiology and pathology. Different approaches have been tried for the primary culture of human IECs, with varying degrees of success, as apoptosis easily occurs. Our aim was to develop a method for primary culture of human IECs from biopsy material. IECs and Lamina propria (LP) cells were liberated from duodenal biopsies obtained from subjects undergoing routine endoscopy for clinical investigations, whose small bowel was macroscopically normal. IECs were cultured on collagen membranes in a 12-well tissue culture cluster, with LP cells and allogeneic Epstein-Barr Virus (EBV)-transformed B lymphocytes (allo-B cells) underneath, in the well. Cultured IECs were characterized by light and confocal microscopy. Cytokine levels in culture supernatants were measured by ELISA. Cells showed the columnar morphology of IECs, even after several days in culture. Best results were obtained from IECs cultured above both LP and allo-B cells. IECs did not form monolayers as do transformed epithelial cell lines, but they did preserve their original cell-cell contacts. Analysis of culture supernatants showed that IL-10 was produced by IECs initially, but IL-1ra was produced by LP cells in the underlying wells with increasing time in culture. Very little IL-1 beta was produced from any cultures. These results show that IECs can be isolated and maintained in primary culture for a short while, which could open new possibilities for research using patient material instead of cell lines.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.