ArticleNucleic acids research2001
The role of DNA polymerase activity in human non-homologous end joining.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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Who cites it
16 citing papers in PubMed, 39 citations in OpenAlex.
- Cellular and Molecular Mechanisms of Oxidative DNA Damage and Repair.Medicina (Kaunas, Lithuania) · 2025Review
- The consequences of viral infection on host DNA damage response: a focus on SARS-CoVs.Journal, genetic engineering & biotechnology · 2022Review
- Chaperoning histones at the DNA repair dance.DNA repair · 2021Review
- DNA Polymerase alpha is essential for intracellular amplification of hepatitis B virus covalently closed circular DNA.PLoS pathogens · 2019Article
- Article
- Caught with One's Zinc Fingers in the Genome Integrity Cookie Jar.Trends in genetics : TIG · 2018Review
- DNA-binding determinants promoting NHEJ by human Polμ.Nucleic acids research · 2012Article
- Biochemical characterization of metnase's endonuclease activity and its role in NHEJ repair.Biochemistry · 2011Article
- Proofreading activity of DNA polymerase Pol2 mediates 3'-end processing during nonhomologous end joining in yeast.PLoS genetics · 2008Article
- Chromatin disassembly and reassembly during DNA repair.Mutation research · 2007Review
- The SET domain protein Metnase mediates foreign DNA integration and links integration to nonhomologous end-joining repair.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- The yeast histone chaperone chromatin assembly factor 1 protects against double-strand DNA-damaging agents.Genetics · 2005Article
- Processing of DNA for nonhomologous end-joining by cell-free extract.The EMBO journal · 2005Article
- Association of DNA polymerase mu (pol mu) with Ku and ligase IV: role for pol mu in end-joining double-strand break repair.Molecular and cellular biology · 2002Article
- Transduction of interphase cells by avian sarcoma virus.Journal of virology · 2002Article
- Human DNA polymerase mu (Pol mu) exhibits an unusual replication slippage ability at AAF lesion.Nucleic acids research · 2002Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In mammalian cells, DNA double-strand breaks are repaired mainly by non-homologous end joining, which modifies and ligates two DNA ends without requiring extensive base pairing interactions for alignment. We investigated the role of DNA polymerases in DNA-PK-dependent end joining of restriction-digested plasmids in vitro and in vivo. Rejoining of DNA blunt ends as well as those with partially complementary 5' or 3' overhangs was stimulated by 20-53% in HeLa cell-free extracts when dNTPs were included, indicating that part of the end joining is dependent on DNA synthesis. This DNA synthesis-dependent end joining was sensitive to aphidicolin, an inhibitor of alpha-like DNA polymerases. Furthermore, antibodies that neutralize the activity of DNA polymerase alpha were found to strongly inhibit end joining in vitro, whereas neutralizing antibodies directed against DNA polymerases beta and epsilon did not. DNA sequence analysis of end joining products revealed two prominent modes of repair, one of which appeared to be dependent on DNA synthesis. Identical products of end joining were recovered from HeLa cells after transfection with one of the model substrates, suggesting that the same end joining mechanisms also operate in vivo. Fractionation of cell extracts to separate PCNA as well as depletion of cell extracts for PCNA resulted in a moderate but significant reduction in end joining activity, suggesting a potential role in a minor repair pathway.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.