ArticleNucleic acids research2001
Effect of large targeted deletions on the mitotic stability of an extra chromosome mediating drug resistance in Leishmania.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed.
- Identification of the centromeres ofEMBO reports · 2017Article
- Genome-wide mapping reveals single-origin chromosome replication in Leishmania, a eukaryotic microbe.Genome biology · 2015Article
- Drug resistance analysis by next generation sequencing in Leishmania.International journal for parasitology. Drugs and drug resistance · 2015Review
- Genome-wide stochastic adaptive DNA amplification at direct and inverted DNA repeats in the parasite Leishmania.PLoS biology · 2014Article
- Artificial chromosome formation in maize (Zea mays L.).Chromosoma · 2009Article
- The SIDER2 elements, interspersed repeated sequences that populate the Leishmania genomes, constitute subfamilies showing chromosomal proximity relationship.BMC genomics · 2008Article
- Modulation of gene expression in drug resistant Leishmania is associated with gene amplification, gene deletion and chromosome aneuploidy.Genome biology · 2008Article
- Functional mapping of a trypanosome centromere by chromosome fragmentation identifies a 16-kb GC-rich transcriptional "strand-switch" domain as a major feature.Genome research · 2005Article
- Formation of linear inverted repeat amplicons following targeting of an essential gene in Leishmania.Nucleic acids research · 2005Article
- The small chromosomes of Trypanosoma brucei involved in antigenic variation are constructed around repetitive palindromes.Genome research · 2004Article
- Physiological consequences of drug resistance in Leishmania and their relevance for chemotherapy.Kinetoplastid biology and disease · 2003Article
- Leishmania major chromosome 3 contains two long convergent polycistronic gene clusters separated by a tRNA gene.Nucleic acids research · 2003Article
- The switch region on Leishmania major chromosome 1 is not required for mitotic stability or gene expression, but appears to be essential.Nucleic acids research · 2002Article
- Metabolic selection of a homologous recombination-mediated gene loss protects Trypanosoma brucei from ROS production by glycosomal fumarate reductase.The Journal of biological chemistryArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
A mitotically stable linear extra chromosome obtained in a Leishmania donovani strain rendered mycophenolic acid-resistant has been physically mapped. This 290-kb chromosome has an inverted duplicated structure around a central inversion region, and is derived from a conservative amplification event of a approximately 140-kb subtelomeric end of chromosome 19. Large-sized targeted deletions of the central region were performed through homologous recombination using three specific transfection vectors. The size of the extra chromosome was thus successfully reduced from 290 to 260, 200 and 120 kb respectively. The mitotic stability of these chromosomes was then analysed in drug-free cultures over >140 days. Results differed according to the deletion created. By contrast with the smallest deletion the two largest deletions altered mitotic stability, leading to progressive loss of the size-reduced chromosomes with similar kinetics in both mutants. The 30-kb region common to both deletions may therefore be considered as involved in mitotic stability. A 44-kb contig covering this region could be assembled and sequenced. The analysis of this sequence did not reveal any sequence elements typical of centromeric DNA. By contrast, its enrichment in homopolymer tracts suggests that this region might contain an origin of replication.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.