ArticleThe Journal of cell biology2001
Targeting of an abundant cytosolic form of the protein import receptor at Toc159 to the outer chloroplast membrane.
Article in The Journal of cell biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 129 citations in OpenAlex.
- Assembly of two functionally-distinct protein import complexes in the outer membrane of plant chloroplasts.Nature communications · 2026Article
- Identification, cloning and expression analysis of chloroplast TOC-TIC complex components in a CBMC plant biology · 2026Article
- Identification of 1,6-hexadecanediol and its wax diesters in chloroplasts of Nicotiana benthamiana.Planta · 2025Article
- Hemolysin-like Protein of 'Microorganisms · 2025Article
- Arabidopsis conditional photosynthesis mutants abc1k1 and var2 accumulate partially processed thylakoid preproteins and are defective in chloroplast biogenesis.Communications biology · 2025Article
- A viral protein targets mitochondria and chloroplasts by subverting general import pathways and specific receptors.Journal of virology · 2023Article
- Understanding protein import in diverse non-green plastids.Frontiers in genetics · 2023Review
- Article
- Molecular characterization, targeting and expression analysis of chloroplast and mitochondrion protein import components inFrontiers in plant science · 2022Article
- Article
- HS1 Is Involved in Hygromycin Resistance Through Facilitating Hygromycin Phosphotransferase Transportation From Cytosol to Chloroplast.Frontiers in plant science · 2020Article
- The novel chloroplast outer membrane kinase KOC1 is a required component of the plastid protein import machinery.The Journal of biological chemistry · 2017Article
- Identification of Plastoglobules as a Site of Carotenoid Cleavage.Frontiers in plant science · 2016Article
- Characterization of chloroplast protein import without Tic56, a component of the 1-megadalton translocon at the inner envelope membrane of chloroplasts.Plant physiology · 2015Article
- Sample preparation for phosphoproteomic analysis of circadian time series in Arabidopsis thaliana.Methods in enzymology · 2015Article
- Targeting of a polytopic membrane protein to the inner envelope membrane of chloroplasts in vivo involves multiple transmembrane segments.Journal of experimental botany · 2014Article
- The C-terminus of Bienertia sinuspersici Toc159 contains essential elements for its targeting and anchorage to the chloroplast outer membrane.Frontiers in plant science · 2014Article
- Border control: selectivity of chloroplast protein import and regulation at the TOC-complex.Frontiers in plant science · 2014Review
- Targeting and assembly of components of the TOC protein import complex at the chloroplast outer envelope membrane.Frontiers in plant science · 2014Review
- A chloroplast ABC1-like kinase regulates vitamin E metabolism in Arabidopsis.Plant physiology · 2013Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chloroplast biogenesis requires the large-scale import of cytosolically synthesized precursor proteins. A trimeric translocon (Toc complex) containing two homologous GTP-binding proteins (atToc33 and atToc159) and a channel protein (atToc75) facilitates protein translocation across the outer envelope membrane. The mechanisms governing function and assembly of the Toc complex are not yet understood. This study demonstrates that atToc159 and its pea orthologue exist in an abundant, previously unrecognized soluble form, and partition between cytosol-containing soluble fractions and the chloroplast outer membrane. We show that soluble atToc159 binds directly to the cytosolic domain of atToc33 in a homotypic interaction, contributing to the integration of atToc159 into the chloroplast outer membrane. The data suggest that the function of the Toc complex involves switching of atToc159 between a soluble and an integral membrane form.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.