ArticleVirchows Archiv : an international journal of pathology2001
Immortalized bovine pancreatic duct cells become tumorigenic after transfection with mutant k-ras.
Article in Virchows Archiv : an international journal of pathology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 9 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed, 1 synthesis or guideline pooled it, 31 citations in OpenAlex.
- Frequency of K-ras mutations in pancreatic intraductal neoplasias associated with pancreatic ductal adenocarcinoma and chronic pancreatitis: a meta-analysis.Neoplasia (New York, N.Y.) · 2005Pooled it
- Review
- Porcine pancreatic ductal epithelial cells transformed with KRASScientific reports · 2021Article
- Article
- Transdifferentiation of tadpole pancreatic acinar cells to duct cells mediated by Notch and stromelysin-3.Developmental biology · 2011Article
- Autoantibodies against the exocrine pancreas in autoimmune pancreatitis: gene and protein expression profiling and immunoassays identify pancreatic enzymes as a major target of the inflammatory process.The American journal of gastroenterology · 2010Article
- Remodeling the exocrine pancreas at metamorphosis in Xenopus laevis.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Genes and proteins differentially expressed during in vitro malignant transformation of bovine pancreatic duct cells.Neoplasia (New York, N.Y.) · 2007Article
- Gene expression profiles of microdissected pancreatic ductal adenocarcinoma.Virchows Archiv : an international journal of pathology · 2003Article
Corrections and comments
- Erratum issuedJesnowski, R [corrected to Jesenofsky, R]
Authors and funding
11 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mutation of the K-ras gene is thought to be an early and important event in pancreatic carcinogenesis. In order to study the role of this molecular alteration in the transition from the normal to the neoplastic pancreatic cell, bovine pancreatic duct cells were first immortalized by SV40 large T antigen (Ag) complementary (c)DNA transfection and then transfected with a mutated K-ras gene. As did primary duct cells, the immortalized duct cells (more than 100 passages) expressed cytokeratins, carbonic anhydrase type-II, cystic fibrosis transmembrane conductance regulator (CFTR), and multidrug resistance (mdr). They grew as a single layer after transplantation under plastic domes and formed three-dimensional structures resembling ducts when grown on Matrigel. Cell growth was stimulated by insulin, epidermal growth factor (EGF), transforming growth factor (TGF)-alpha, but cells did not respond to gastrin and CCK-8. They did not form colonies in soft agar nor did they form tumors in nude mice. Immortalized cells transfected with mutated K-ras acquired the ability to form tumors after orthotopic injection into the nude mouse pancreas. It is concluded that SV 40 immortalized bovine pancreatic
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