ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2001
Gap junctions mediate electrical signaling and ensuing cytosolic Ca2+ increases between chromaffin cells in adrenal slices: A role in catecholamine release.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 95 citations in OpenAlex.
- Defining the electrical synapse.Nature reviews. Neuroscience · 2026Review
- Influence of extracellular magnesium ions on the functional state and biophysical and structural properties of connexin36 hemichannels.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Expression and function of Connexin 43 and Connexin 37 in the murine zona glomerulosa.Physiological reports · 2025Article
- Early life microbiome disbalance impacts neuroendocrine outcomes in pre-pubertal mice in a sexually dimorphic manner.Frontiers in microbiology · 2025Article
- Adaptive remodeling of rat adrenomedullary stimulus-secretion coupling in a chronic hypertensive environment.Cellular and molecular life sciences : CMLS · 2024Article
- Developmental Changes in Gap Junction Expression in Rat Adrenal Medullary Chromaffin Cells.Acta histochemica et cytochemica · 2024Article
- The Role of the Gap Junction Protein Connexin in Adrenal Gland Tumorigenesis.International journal of molecular sciences · 2024Review
- Article
- Calcium Imaging and Amperometric Recording in Cultured Chromaffin Cells and Adrenal Slices from Normotensive, Wistar Kyoto Rats and Spontaneously Hypertensive Rats.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Basal and Stress-Induced Network Activity in the Adrenal MedullaFrontiers in endocrinology · 2022Article
- A sodium background conductance controls the spiking pattern of mouse adrenal chromaffin cells in situ.The Journal of physiology · 2021Article
- Chromaffin Cells of the Adrenal Medulla: Physiology, Pharmacology, and Disease.Comprehensive Physiology · 2019Article
- Gap junction communication between chromaffin cells: the hidden face of adrenal stimulus-secretion coupling.Pflugers Archiv : European journal of physiology · 2018Review
- Old and emerging concepts on adrenal chromaffin cell stimulus-secretion coupling.Pflugers Archiv : European journal of physiology · 2018Article
- What's New in Endocrinology: The Chromaffin Cell.Frontiers in endocrinology · 2018Review
- Two Forms of Electrical Transmission Between Neurons.Frontiers in molecular neuroscience · 2018Article
- Spatially Organized β-Cell Subpopulations Control Electrical Dynamics across Islets of Langerhans.Biophysical journal · 2017Article
- Connexin36 localization to pinealocytes in the pineal gland of mouse and rat.The European journal of neuroscience · 2017Article
- Monitoring the Secretory Behavior of the Rat Adrenal Medulla by High-Performance Liquid Chromatography-Based Catecholamine Assay from Slice Supernatants.Frontiers in endocrinology · 2017Article
- A high-throughput fluorimetric microarray with enhanced fluorescence and suppressed "coffee-ring" effects for the detection of calcium ions in blood.Scientific reports · 2016Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In adrenal chromaffin cells, a rise in cytosolic calcium concentration ([Ca(2+)]i) is a key event in the triggering of catecholamine exocytosis after splanchnic nerve activation. Action potential- or nicotine-induced [Ca(2+)]i transients are well described in individual chromaffin cells, but whether they remain spatially confined to the stimulated cell or propagate to adjacent cells is not yet known. To address this issue, the spatiotemporal organization of electrical and associated Ca(2+) events between chromaffin cells was investigated using the patch-clamp technique and real-time confocal imaging in rat acute adrenal slices. Spontaneous or electrically evoked action potential-driven [Ca(2+)]i transients were simultaneously detected in neighboring cells. This was likely attributable to gap junction-mediated electrotonic communication, as shown by (1) the bidirectional reflection of voltage changes monitored between cell pairs, (2) Lucifer yellow (LY) diffusion between cells exhibiting spontaneous synchronized [Ca(2+)]i transients, and (3) the reduction of LY diffusion using the uncoupling agent carbenoxolone. Furthermore, transcripts encoding two connexins (Cx36 and Cx43) were found in single chromaffin cells. This gap junctional coupling was activated after a synaptic-like application of nicotine that mediated synchronous multicellular [Ca(2+)]i increases. In addition, nicotinic stimulation of a single cell triggered catecholamine release in coupled cells, as shown by amperometric detection of secretory events. Functional coupling between chromaffin cells in situ may represent an efficient complement to synaptic transmission to amplify catecholamine release after synaptic stimulation of a single excited chromaffin cell.
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Identifiers
11466411PMC6762648W2111947024What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.