ArticleJournal of bacteriology2001
Requirement of novel competence genes pilT and pilU of Pseudomonas stutzeri for natural transformation and suppression of pilT deficiency by a hexahistidine tag on the type IV pilus protein PilAI.
Article in Journal of bacteriology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Discovery of PilU as a second type IV pilus retraction motor inJournal of bacteriology · 2026Article
- Comparative genomics ofFrontiers in microbiology · 2023Article
- Retraction ATPase Motors from Three Orthologous Type IVa Pilus Systems Support Promiscuous Retraction of the Vibrio cholerae Competence Pilus.Journal of bacteriology · 2022Article
- Natural transformation in Gram-negative bacteria thriving in extreme environments: from genes and genomes to proteins, structures and regulation.Extremophiles : life under extreme conditions · 2021Review
- The Role of the Cyanobacterial Type IV Pilus Machinery in Finding and Maintaining a Favourable Environment.Life (Basel, Switzerland) · 2020Review
- The type IV pilus protein PilU functions as a PilT-dependent retraction ATPase.PLoS genetics · 2019Article
- DNA Uptake by Type IV Filaments.Frontiers in molecular biosciences · 2019Review
- Uptake of extracellular DNA: competence induced pili in natural transformation of Streptococcus pneumoniae.BioEssays : news and reviews in molecular, cellular and developmental biology · 2015Article
- Type IV pilus biogenesis, twitching motility, and DNA uptake in Thermus thermophilus: discrete roles of antagonistic ATPases PilF, PilT1, and PilT2.Applied and environmental microbiology · 2014Article
- Sequence of the hyperplastic genome of the naturally competent Thermus scotoductus SA-01.BMC genomics · 2011Article
- Systematic functional analysis reveals that a set of seven genes is involved in fine-tuning of the multiple functions mediated by type IV pili in Neisseria meningitidis.Infection and immunity · 2010Article
- Twitching motility is essential for virulence in Dichelobacter nodosus.Journal of bacteriology · 2008Article
- The outer membrane secretin PilQ from Neisseria meningitidis binds DNA.Microbiology (Reading, England) · 2007Article
- Functional analysis of PilT from the toxic cyanobacterium Microcystis aeruginosa PCC 7806.Journal of bacteriology · 2007Article
- Biology of Pseudomonas stutzeri.Microbiology and molecular biology reviews : MMBR · 2006Review
- Demonstration of Type IV pilus expression and a twitching phenotype by Haemophilus influenzae.Infection and immunity · 2005Article
- DNA binding: a novel function of Pseudomonas aeruginosa type IV pili.Journal of bacteriology · 2005Article
- Disparate subcellular localization patterns of Pseudomonas aeruginosa Type IV pilus ATPases involved in twitching motility.Journal of bacteriology · 2005Article
- DNA transport and natural transformation in mesophilic and thermophilic bacteria.Journal of bioenergetics and biomembranes · 2004Review
- Pilin-like proteins in the extremely thermophilic bacterium Thermus thermophilus HB27: implication in competence for natural transformation and links to type IV pilus biogenesis.Applied and environmental microbiology · 2003Article
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4 authors.
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Abstract
The ubiquitous species Pseudomonas stutzeri has type IV pili, and these are essential for the natural transformation of the cells. An absolute transformation-deficient mutant obtained after transposon mutagenesis had an insertion in a gene which was termed pilT. The deduced amino acid sequence has identity with PilT of Pseudomonas aeruginosa (94%), Neisseria gonorrhoeae (67%), and other gram-negative species and it contains a nucleotide-binding motif. The mutant was hyperpiliated but defective for further pilus-associated properties, such as twitching motility and plating of pilus-specific phage PO4. [(3)H]thymidine-labeled DNA was bound by the mutant but not taken up. Downstream of pilT a gene, termed pilU, coding for a putative protein with 88% amino acid identity with PilU of P. aeruginosa was identified. Insertional inactivation did not affect piliation, twitching motility, or PO4 infection but reduced transformation to about 10%. The defect was fully complemented by PilU of nontransformable P. aeruginosa. When the pilAI gene (coding for the type IV pilus prepilin) was manipulated to code for a protein in which the six C-terminal amino acids were replaced by six histidine residues and then expressed from a plasmid, it gave a nonpiliated and twitching motility-defective phenotype in pilAI::Gm(r) cells but allowed transformability. Moreover, the mutant allele suppressed the absolute transformation deficiency caused by the pilT mutation. Considering the hypothesized role of pilT(+) in pilus retraction and the presumed requirement of retraction for DNA uptake, it is proposed that the pilT-independent transformation is promoted by PilA mutant protein either as single molecules or as minimal pilin assembly structures in the periplasm which may resemble depolymerized pili and that these cause the outer membrane pores to open for DNA entry.
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