ArticleThe British journal of ophthalmology2001
In situ immunohistochemical study of Bcl-2 and heat shock proteins in human corneal endothelial cells during corneal storage.
Article in The British journal of ophthalmology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 20 citations in OpenAlex.
- Molecular Chaperone Hsp70 and Its Constitutively Active Form Hsc70 Play an Indispensable Role During Eye Development of Drosophila melanogaster.Molecular neurobiology · 2018Article
- Article
- Impact of temporary hyperthermia on corneal endothelial cell survival during organ culture preservation.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2015Article
- Donor cornea transfer from Optisol GS to organ culture storage: a two-step procedure to increase donor tissue lifespan.Acta ophthalmologica · 2013Article
- Impact of diabetes on alpha-crystallins and other heat shock proteins in the eye.Journal of ocular biology, diseases, and informatics · 2011Article
- Corneal endothelial cells are protected from apoptosis by gene therapy.Human gene therapy · 2011Article
- Cigarette smoke-related hydroquinone induces filamentous actin reorganization and heat shock protein 27 phosphorylation through p38 and extracellular signal-regulated kinase 1/2 in retinal pigment epithelium: implications for age-related macular degeneration.The American journal of pathology · 2010Article
- Assessment of heat shock protein (HSP60, HSP72, HSP90, and HSC70) expression in cultured limbal stem cells following air lifting.Molecular vision · 2010Article
- Heat shock proteins in the human eye.International journal of proteomics · 2010Article
- Construction of human liver cancer vascular endothelium cDNA expression library and screening of the endothelium-associated antigen genes.World journal of gastroenterology · 2004Article
- Mechanisms of staurosporine induced apoptosis in a human corneal endothelial cell line.The British journal of ophthalmology · 2003Article
- Value of two mortality assessment techniques for organ cultured corneal endothelium: trypan blue versus TUNEL technique.The British journal of ophthalmology · 2002Article
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Authors and funding
6 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo investigate the expression of Bcl-2 and heat shock proteins (HSPs), which are known to increase cell survival, in human corneal endothelial cells (HCECs) of corneas stored in organ culture.
methods32 paired corneas were randomly assigned to either a short or a long storage time. The flat mounts of endothelium were examined after immunostaining with monoclonal antibodies to Bcl-2 and HSP 27, 60, 70, and 90.
resultsHCECs expressed generally all the proteins studied. Bcl-2 expression was weaker in the long stored corneas (p=0.035). There was no relation between immunostaining, age, sex, or death to culture time. Frequently some Descemet membranes carried negative cells preferentially located in folds and exhibiting morphological changes consistent with swelling cells corresponding to early stages of apoptosis.
conclusionExpression of these cytoprotective proteins reflects the high level of HCEC resistance to stresses induced by organ culture. The decreased immunostaining of Bcl-2 in the long storage group could act in cellular loss currently observed with storage time. The negativity of Bcl-2 and HSP labelling in corneal folding may be related to apoptosis.
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