ArticleOncogene2001
A repetitive element containing a critical tyrosine residue is required for transcriptional activation by the EWS/ATF1 oncogene.
Article in Oncogene, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 29 citations in OpenAlex.
- Review
- A PITX2-HTR1B pathway regulates the asymmetric development of female gonads in chickens.PNAS nexus · 2023Article
- RGG boxes within the TET/FET family of RNA-binding proteins are functionally distinct.Transcription · 2016Article
- Polycation-π interactions are a driving force for molecular recognition by an intrinsically disordered oncoprotein family.PLoS computational biology · 2013Article
- Article
- A transcription assay for EWS oncoproteins in Xenopus oocytes.Protein & cell · 2010Article
- In vitro interaction between the N-terminus of the Ewing's sarcoma protein and the subunit of RNA polymerase II hsRPB7.Molecular biology reports · 2009Article
- A single aromatic residue in transcriptional repressor protein KorA is critical for cooperativity with its co-regulator KorB.Molecular microbiology · 2008Article
- Multiple aromatic side chains within a disordered structure are critical for transcription and transforming activity of EWS family oncoproteins.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- RGG-boxes of the EWS oncoprotein repress a range of transcriptional activation domains.Nucleic acids research · 2005Article
- Homotypic and heterotypic interactions of EWS, FLI1 and their oncogenic fusion protein.Oncogene · 2003Article
- The melanocyte inducing factor MITF is stably expressed in cell lines from human clear cell sarcoma.British journal of cancer · 2003Article
- Molecular diagnosis of clear cell sarcoma: detection of EWS-ATF1 and MITF-M transcripts and histopathological and ultrastructural analysis of 12 cases.The Journal of molecular diagnostics : JMD · 2002Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chromosomal fusion of the N-terminal region of the Ewings Sarcoma Oncogene (EWS-activation-domain, EAD) to the DNA-binding domains of a variety of cellular transcription factors produce oncogenic proteins (EWS-fusion proteins (EFPs)) that cause distinct malignancies. In EFPs, the EAD acts as a potent transcriptional activation domain and this ability is repressed in the context of normal, non-tumorigenic, EWS. Trans-activation by the EAD is therefore a specific characteristic of EFPs and it is thought that EFPs induce tumorigenesis via improper transcriptional activation of cellular genes. Functional elements required for transcriptional activation are dispersed throughout the EAD, as are thirty-one copies of a Degenerate Hexapeptide Repeat (DHR, consensus SYGQQS). This suggests that the EAD contains a highly reiterated functional element related to DHRs. Here we show that in the context of EWS/ATF1, the EFP that causes malignant melanoma of soft parts, trans-cooperation by small regions of the EAD ( approximately 30 residues) results in potent transcriptional activation dependent on the conserved tyrosine residues present in DHRs. These findings provide the first evidence for a role of DHRs in EAD-mediated trans-activation and demonstrate that the EAD represents a novel tyrosine-dependent transcriptional activation domain.
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