ArticleMolecular and cellular biology2001
Yeast RNA polymerase I enhancer is dispensable for transcription of the chromosomal rRNA gene and cell growth, and its apparent transcription enhancement from ectopic promoters requires Fob1 protein.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 41 citations in OpenAlex.
- New insights into nuclear import and nucleolar localization of yeast RNA exosome subunits.Molecular biology of the cell · 2025Article
- Proteins and RNA sequences required for the transition of the t-Utp complex into the SSU processome.FEMS yeast research · 2019Article
- Compositional and structural analysis of selected chromosomal domains from Saccharomyces cerevisiae.Nucleic acids research · 2014Article
- Co-transcriptional RNA cleavage provides a failsafe termination mechanism for yeast RNA polymerase I.Nucleic acids research · 2011Article
- Review
- Actively transcribed rRNA genes in S. cerevisiae are organized in a specialized chromatin associated with the high-mobility group protein Hmo1 and are largely devoid of histone molecules.Genes & development · 2008Article
- Unreplicated DNA in mitosis precludes condensin binding and chromosome condensation in S. cerevisiae.Frontiers in bioscience : a journal and virtual library · 2008Article
- Budding yeast RNA polymerases I and II employ parallel mechanisms of transcriptional termination.Genes & development · 2008Article
- Replication fork barriers: pausing for a break or stalling for time?EMBO reports · 2007Review
- Expression of rRNA genes and nucleolus formation at ectopic chromosomal sites in the yeast Saccharomyces cerevisiae.Molecular and cellular biology · 2006Article
- Yeast nuclear envelope subdomains with distinct abilities to resist membrane expansion.Molecular biology of the cell · 2006Article
- DEG1, encoding the tRNA:pseudouridine synthase Pus3p, impacts HOT1-stimulated recombination in Saccharomyces cerevisiae.Molecular genetics and genomics : MGG · 2005Article
- Identifying gene-independent noncoding functional elements in the yeast ribosomal DNA by phylogenetic footprinting.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Spontaneous rDNA copy number variation modulates Sir2 levels and epigenetic gene silencing.Genes & development · 2005Article
- SCH9, a putative protein kinase from Saccharomyces cerevisiae, affects HOT1-stimulated recombination.Molecular genetics and genomics : MGG · 2004Article
- Transcription termination factor reb1p causes two replication fork barriers at its cognate sites in fission yeast ribosomal DNA in vivo.Molecular and cellular biology · 2004Article
- The replication fork barrier site forms a unique structure with Fob1p and inhibits the replication fork.Molecular and cellular biology · 2003Article
- Association of the RENT complex with nontranscribed and coding regions of rDNA and a regional requirement for the replication fork block protein Fob1 in rDNA silencing.Genes & development · 2003Article
- Transcription-dependent recombination and the role of fork collision in yeast rDNA.Genes & development · 2003Article
- Mutations in DNA replication genes reduce yeast life span.Molecular and cellular biology · 2002Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 2 countries.
Funding
Abstract
At the end of the 35S rRNA gene within ribosomal DNA (rDNA) repeats in Saccharomyces cerevisiae lies an enhancer that has been shown to greatly stimulate rDNA transcription in ectopic reporter systems. We found, however, that the enhancer is not necessary for normal levels of rRNA synthesis from chromosomal rDNA or for cell growth. Yeast strains which have the entire enhancer from rDNA deleted did not show any defects in growth or rRNA synthesis. We found that the stimulatory activity of the enhancer for ectopic reporters is not observed in cells with disrupted nucleolar structures, suggesting that reporter genes are in general poorly accessible to RNA polymerase I (Pol I) machinery in the nucleolus and that the enhancer improves accessibility. We also found that a fob1 mutation abolishes transcription from the enhancer-dependent rDNA promoter integrated at the HIS4 locus without any effect on transcription from chromosomal rDNA. FOB1 is required for recombination hot spot (HOT1) activity, which also requires the enhancer region, and for recombination within rDNA repeats. We suggest that Fob1 protein stimulates interactions between rDNA repeats through the enhancer region, thus helping ectopic rDNA promoters to recruit the Pol I machinery normally present in the nucleolus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.