ArticleMolecular and cellular biology2001
High-copy-number expression of Sub2p, a member of the RNA helicase superfamily, suppresses hpr1-mediated genomic instability.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 54 citations in OpenAlex.
- Tho1 and MOS11 promote nucleic acid double-strand unwinding by facilitating DEAD-box helicase oligomerization.Nucleic acids research · 2026Article
- Structures and mRNP remodeling mechanism of the TREX-2 complex.Structure (London, England : 1993) · 2025Article
- The Hog1 MAPK substrate governs Candida glabrata-epithelial cell adhesion via the histone H2A variant.PLoS genetics · 2024Article
- Native functions of short tandem repeats.eLife · 2023Article
- DEAD-Box RNA Helicases and Genome Stability.Genes · 2021Review
- Physical proximity of chromatin to nuclear pores prevents harmful R loop accumulation contributing to maintain genome stability.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- HYPER RECOMBINATION1 of the THO/TREX complex plays a role in controlling transcription of the REVERSION-TO-ETHYLENE SENSITIVITY1 gene in Arabidopsis.PLoS genetics · 2015Article
- Transcription and recombination: when RNA meets DNA.Cold Spring Harbor perspectives in biology · 2014Review
- Telomeric transcripts stimulate telomere recombination to suppress senescence in cells lacking telomerase.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- The Npl3 hnRNP prevents R-loop-mediated transcription-replication conflicts and genome instability.Genes & development · 2013Article
- Mutational analysis of the yeast RNA helicase Sub2p reveals conserved domains required for growth, mRNA export, and genomic stability.RNA (New York, N.Y.) · 2013Article
- Article
- R-loops do not accumulate in transcription-defective hpr1-101 mutants: implications for the functional role of THO/TREX.Nucleic acids research · 2009Article
- Thoc1 deficiency compromises gene expression necessary for normal testis development in the mouse.Molecular and cellular biology · 2009Article
- The THP1-SAC3-SUS1-CDC31 complex works in transcription elongation-mRNA export preventing RNA-mediated genome instability.Molecular biology of the cell · 2008Article
- Review
- Different physiological relevance of yeast THO/TREX subunits in gene expression and genome integrity.Molecular genetics and genomics : MGG · 2008Article
- The RNA binding protein RNPS1 alleviates ASF/SF2 depletion-induced genomic instability.RNA (New York, N.Y.) · 2007Article
- An hpr1 point mutation that impairs transcription and mRNP biogenesis without increasing recombination.Molecular and cellular biology · 2006Article
- Genes with internal repeats require the THO complex for transcription.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We report on a novel role for a pre-mRNA splicing component in genome stability. The Hpr1 protein, a component of an RNA polymerase II complex and required for transcription elongation, is also required for genome stability. Deletion of HPR1 results in a 1,000-fold increase in genome instability, detected as direct-repeat instability. This instability can be suppressed by the high-copy-number SUB2 gene, which is the Saccharomyces cerevisiae homologue of the human splicing factor hUAP56. Although SUB2 is essential, conditional alleles grown at the permissive temperature complement the essential function of SUB2 yet reveal nonessential phenotypes. These studies have uncovered a role for SUB2 in preventing genome instability. The genomic instability observed in sub2 mutants can be suppressed by high-copy-number HPR1. A deletion mutant of CDC73, a component of a PolII complex, is also unstable for direct repeats. This too is suppressed by high-copy-number SUB2. Thus, defects in both the transcriptional machinery and the pre-mRNA splicing machinery can be sources of genome instability. The ability of a pre-mRNA splicing factor to suppress the hyperrecombination phenotype of a defective PolII complex raises the possibility of integrating transcription, RNA processing, and genome stability or a second role for SUB2.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.