ArticleMolecular and cellular biology2001
Polynucleotide phosphorylase functions as both an exonuclease and a poly(A) polymerase in spinach chloroplasts.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
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73 citing papers in PubMed, 164 citations in OpenAlex.
- Distribution of poly(A) polymerase I in bacteria: an expanded role for horizontal gene transfer.Microbiology (Reading, England) · 2026Article
- Dysregulated 3'-end processing of 18S pre-rRNA decreases mtPNPase efficiency in plant mitochondria.Nucleic acids research · 2026Article
- RNA exoribonucleases in E. coli.Archives of microbiology · 2026Review
- An Addendum to the Chemiosmotic Theory of Mitochondrial Activity: The Role of RNA as a Proton Sink.Biomolecules · 2025Review
- Overexpression of a pseudo-etiolated-in-light-like protein inFrontiers in plant science · 2023Article
- Integrating physiology, genetics, and transcriptome to decipher a new thermo-sensitive and light-sensitive virescent leaf gene mutant in cucumber.Frontiers in plant science · 2022Article
- Review
- CRISPR-based DNA and RNA detection with liquid-liquid phase separation.Biophysical journal · 2021Article
- Acquisition ofMicrobial genomics · 2021Article
- Organellar and Secretory Ribonucleases: Major Players in Plant RNA Homeostasis.Plant physiology · 2020Article
- Article
- Nonspherical Coacervate Shapes in an Enzyme-Driven Active System.Langmuir : the ACS journal of surfaces and colloids · 2020Article
- Proteomic Response ofBiomolecules · 2019Article
- Phylogeny and Evolution of RNA 3'-Nucleotidyltransferases in Bacteria.Journal of molecular evolution · 2019Article
- Spatiotemporal control of coacervate formation within liposomes.Nature communications · 2019Article
- Polynucleotide phosphorylase: Not merely an RNase but a pivotal post-transcriptional regulator.PLoS genetics · 2018Review
- Polyadenylation and degradation of structurally abnormal mitochondrial tRNAs in human cells.Nucleic acids research · 2018Article
- Novel Aspects of Polynucleotide Phosphorylase Function in Streptomyces.Antibiotics (Basel, Switzerland) · 2018Review
- RNA degradation by the plant RNA exosome involves both phosphorolytic and hydrolytic activities.Nature communications · 2017Article
- Molecular determinants for CRISPR RNA maturation in the Cas10-Csm complex and roles for non-Cas nucleases.Nucleic acids research · 2017Article
13 more citing papers are in PubMed but not listed here.
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3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The molecular mechanism of mRNA degradation in the chloroplast consists of sequential events including endonucleolytic cleavage, the addition of poly(A)-rich sequences to the endonucleolytic cleavage products, and exonucleolytic degradation by polynucleotide phosphorylase (PNPase). In Escherichia coli, polyadenylation is performed mainly by poly(A)-polymerase (PAP) I or by PNPase in its absence. While trying to purify the chloroplast PAP by following in vitro polyadenylation activity, it was found to copurify with PNPase and indeed could not be separated from it. Purified PNPase was able to polyadenylate RNA molecules with an activity similar to that of lysed chloroplasts. Both activities use ADP much more effectively than ATP and are inhibited by stem-loop structures. The activity of PNPase was directed to RNA degradation or polymerization by manipulating physiologically relevant concentrations of P(i) and ADP. As expected of a phosphorylase, P(i) enhanced degradation, whereas ADP inhibited degradation and enhanced polymerization. In addition, searching the complete Arabidopsis genome revealed several putative PAPs, none of which were preceded by a typical chloroplast transit peptide. These results suggest that there is no enzyme similar to E. coli PAP I in spinach chloroplasts and that polyadenylation and exonucleolytic degradation of RNA in spinach chloroplasts are performed by one enzyme, PNPase.
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