ArticleMolecular and cellular biology2001
Hic-5-reduced cell spreading on fibronectin: competitive effects between paxillin and Hic-5 through interaction with focal adhesion kinase.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 95 citations in OpenAlex.
- A comparative analysis of paxillin and Hic-5 proximity interactomes.Cytoskeleton (Hoboken, N.J.) · 2025Article
- Physiological and pathological roles of Hic‑5 in several organs (Review).International journal of molecular medicine · 2022Review
- Targeting Src-Hic-5 Signal Cascade for Preventing Migration of Cholangiocarcinoma Cell HuCCT1.Biomedicines · 2022Article
- Paxillin family of focal adhesion adaptor proteins and regulation of cancer cell invasion.International review of cell and molecular biology · 2020Article
- The Leptin induced Hic-5 expression and actin puncta formation by the FAK/Src-dependent pathway in MCF10A mammary epithelial cellsBiomedica : revista del Instituto Nacional de Salud · 2019Article
- Hic-5 regulates Src-induced invadopodia rosette formation and organization.Molecular biology of the cell · 2019Article
- A mitochondrial ROS pathway controls matrix metalloproteinase 9 levels and invasive properties in RAS-activated cancer cells.The FEBS journal · 2019Article
- MicroRNA-29a activates a multi-component growth and invasion program in glioblastoma.Journal of experimental & clinical cancer research : CR · 2019Article
- Review
- Hic-5 regulates epithelial to mesenchymal transition in ovarian cancer cells in a TGFβ1-independent manner.Oncotarget · 2017Article
- Article
- Evolution and Expression of Paxillin Genes in Teleost Fish.PloS one · 2016Article
- Non-receptor tyrosine kinases and the actin cytoskeleton in contractile vascular smooth muscle.The Journal of physiology · 2015Review
- Hic-5 Regulates Actin Cytoskeletal Reorganization and Expression of Fibrogenic Markers and Myocilin in Trabecular Meshwork Cells.Investigative ophthalmology & visual science · 2015Article
- Delineating the Tes Interaction Site in Zyxin and Studying Cellular Effects of Its Disruption.PloS one · 2015Article
- A HIC-5- and KLF4-dependent mechanism transactivates p21(Cip1) in response to anchorage loss.The Journal of biological chemistry · 2012Article
- Expression of phosphorylated heat shock protein 27 during corneal epithelial wound healing.Cornea · 2012Article
- PTTG induces EMT through integrin αVβ3-focal adhesion kinase signaling in lung cancer cells.Oncogene · 2012Article
- Diverse roles for the paxillin family of proteins in cancer.Genes & cancer · 2012Article
- HIC-5: A Mobile Molecular Scaffold Regulating the Anchorage Dependence of Cell Growth.International journal of cell biology · 2012Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hic-5 is a paxillin homologue that is localized to focal adhesion complexes. Hic-5 and paxillin share structural homology and interacting factors such as focal adhesion kinase (FAK), Pyk2/CAKbeta/RAFTK, and PTP-PEST. Here, we showed that Hic-5 inhibits integrin-mediated cell spreading on fibronectin in a competitive manner with paxillin in NIH 3T3 cells. The overexpression of Hic-5 sequestered FAK from paxillin, reduced tyrosine phosphorylation of paxillin and FAK, and prevented paxillin-Crk complex formation. In addition, Hic-5-mediated inhibition of spreading was not observed in mouse embryo fibroblasts (MEFs) derived from FAK(-/-) mice. The activity of c-Src following fibronectin stimulation was decreased by about 30% in Hic-5-expressing cells, and the effect of Hic-5 was restored by the overexpression of FAK and the constitutively active forms of Rho-family GTPases, Rac1 V12 and Cdc42 V12, but not RhoA V14. These observations suggested that Hic-5 inhibits cell spreading through competition with paxillin for FAK and subsequent prevention of downstream signal transduction. Moreover, expression of antisense Hic-5 increased spreading in primary MEFs. These results suggested that the counterbalance of paxillin and Hic-5 expression may be a novel mechanism regulating integrin-mediated signal transduction.
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