ArticleEMBO reports2001
FRET-based detection of different conformations of MK2.
Article in EMBO reports, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 59 citations in OpenAlex.
- Unravelling molecular dynamics in living cells: Fluorescent protein biosensors for cell biology.Journal of microscopy · 2025Review
- Phosphorylation of S-S-S Motif in Nuclear Export Protein (NEP) Plays a Critical Role in Viral Ribonucleoprotein (vRNP) Nuclear Export of Influenza A and B Viruses.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Genetically Encoded Fluorescent Biosensors Illuminate the Spatiotemporal Regulation of Signaling Networks.Chemical reviews · 2018Review
- Targeting Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2, MK2): Medicinal Chemistry Efforts To Lead Small Molecule Inhibitors to Clinical Trials.Journal of medicinal chemistry · 2016Review
- Genetically encoded fluorescent biosensors for live-cell visualization of protein phosphorylation.Chemistry & biology · 2014Review
- Toward a systems-level view of dynamic phosphorylation networks.Frontiers in genetics · 2014Review
- Mapping the phosphoproteome of influenza A and B viruses by mass spectrometry.PLoS pathogens · 2012Article
- Genetically encodable fluorescent biosensors for tracking signaling dynamics in living cells.Chemical reviews · 2011Review
- Activation and function of the MAPKs and their substrates, the MAPK-activated protein kinases.Microbiology and molecular biology reviews : MMBR · 2011Review
- MAPKAP kinase MK2 maintains self-renewal capacity of haematopoietic stem cells.The EMBO journal · 2009Article
- Visualizing and quantifying adhesive signals.Current opinion in cell biology · 2008Review
- Molecular basis of MAPK-activated protein kinase 2:p38 assembly.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Scaffolding by ERK3 regulates MK5 in development.The EMBO journal · 2004Article
- ERK and p38 MAPK-activated protein kinases: a family of protein kinases with diverse biological functions.Microbiology and molecular biology reviews : MMBR · 2004Review
- Signalling takes control of nucleo-cytoplasmic trafficking. Workshop on signal-regulated nuclear transport.EMBO reports · 2002Article
- Molecular biology of stress responses.Cell stress & chaperones · 2002Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MAP kinase-activated protein kinase 2 (MK2 or MAPKAP K2) is a stress-activated enzyme downstream to p38 MAPK. By fusion of green fluorescent protein variants to the N- and C-terminus we analysed conformational changes in the kinase molecule in vitro and in vivo. Activation of MK2 is accompanied by a decrease in fluorescence resonance energy transfer, indicating a transition from an inactive/closed to an active/open conformation with an increase in the apparent distance between the fluorophores of approximately 9 A. The closed conformation exists exclusively in the nucleus. Upon stress, the open conformation of MK2 rapidly becomes detectable in the cytoplasm and accumulates in the nucleus only when Crm1-dependent nuclear export is blocked. Hence, in living cells activation of MK2 and its nuclear export are coupled by a phosphorylation-dependent conformational switch.
Indexed as
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.