ArticleBiophysical journal2001
Atomic force microscopy studies of ganglioside GM1 domains in phosphatidylcholine and phosphatidylcholine/cholesterol bilayers.
Article in Biophysical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 134 citations in OpenAlex.
- Understanding Aβ Peptide Binding to Lipid Membranes: A Biophysical Perspective.International journal of molecular sciences · 2024Review
- Is cholesterol both the lock and key to abnormal transmembrane signals in Autism Spectrum Disorder?Lipids in health and disease · 2024Article
- Structural determinants of REMORIN nanodomain formation in anionic membranes.Biophysical journal · 2023Article
- The glycolipid GM1 reshapes asymmetric biomembranes and giant vesicles by curvature generation.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Diffusion of lipids and GPI-anchored proteins in actin-free plasma membrane vesicles measured by STED-FCS.Molecular biology of the cell · 2017Article
- GM1 Softens POPC Membranes and Induces the Formation of Micron-Sized Domains.Biophysical journal · 2016Article
- Lipid Phase Separation and Protein-Ganglioside Clustering in Supported Bilayers Are Induced by Photorelease of Ceramide.Soft matter · 2013Article
- Single plasmonic nanoparticle tracking studies of solid supported bilayers with ganglioside lipids.Journal of the American Chemical Society · 2012Article
- Part I: an x-ray scattering study of cholera toxin penetration and induced phase transformations in lipid membranes.Biophysical journal · 2008Article
- Asymmetric structural features in single supported lipid bilayers containing cholesterol and GM1 resolved with synchrotron X-Ray reflectivity.Biophysical journal · 2008Article
- GM1 clustering inhibits cholera toxin binding in supported phospholipid membranes.Journal of the American Chemical Society · 2007Article
- Using monomolecular films to characterize lipid lateral interactions.Methods in molecular biology (Clifton, N.J.) · 2007Article
- In situ scanning probe microscopy studies of tetanus toxin-membrane interactions.Biophysical journal · 2006Article
- Galactosylceramide domain microstructure: impact of cholesterol and nucleation/growth conditions.Biophysical journal · 2006Article
- Gating and conductance changes in BK(Ca) channels in bilayers are reciprocal.The Journal of membrane biology · 2006Article
- Local mobility in lipid domains of supported bilayers characterized by atomic force microscopy and fluorescence correlation spectroscopy.Biophysical journal · 2005Article
- Cholera toxin assault on lipid monolayers containing ganglioside GM1.Biophysical journal · 2004Article
- Bilayer thickness modulates the conductance of the BK channel in model membranes.Biophysical journal · 2004Article
- Distribution of GD3 in DPPC monolayers: a thermodynamic and atomic force microscopy combined study.Biophysical journal · 2004Article
- Sphingomyelin/phosphatidylcholine/cholesterol phase diagram: boundaries and composition of lipid rafts.Biophysical journal · 2003Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The distribution of ganglioside in supported lipid bilayers has been studied by atomic force microscopy. Hybrid dipalmitoylphosphatidylcholine (DPPC)/dipalmitoylphosphatidylethanolamine (DPPE) and (2:1 DPPC/cholesterol)/DPPE bilayers were prepared using the Langmuir Blodgett technique. Egg PC and DPPC bilayers were prepared by vesicle fusion. Addition of ganglioside GM1 to each of the lipid bilayers resulted in the formation of heterogeneous surfaces that had numerous small raised domains (30--200 nm in diameter). Incubation of these bilayers with cholera toxin B subunit resulted in the detection of small protein aggregates, indicating specific binding of the protein to the GM1-rich microdomains. Similar results were obtained for DPPC, DPPC/cholesterol, and egg PC, demonstrating that the overall bilayer morphology was not dependent on the method of bilayer preparation or the fluidity of the lipid mixture. However, bilayers produced by vesicle fusion provided evidence for asymmetrically distributed GM1 domains that probably reflect the presence of ganglioside in both inner and outer monolayers of the initial vesicle. The results are discussed in relation to recent inconsistencies in the estimation of sizes of lipid rafts in model and natural membranes. It is hypothesized that small ganglioside-rich microdomains may exist within larger ordered domains in both natural and model membranes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.