ArticleBiophysical journal2001
Phloretin-induced changes of lipophilic ion transport across the plasma membrane of mammalian cells.
Article in Biophysical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 34 citations in OpenAlex.
- The Multi-Conductivity Clausius-Mossotti Factor as an Electrophysiology Rosetta Stone: Dielectrophoresis, Membrane Potential and Zeta Potential.Micromachines · 2025Review
- Label-free, non-contact determination of resting membrane potential using dielectrophoresis.Scientific reports · 2024Article
- Identification of bioactive components behind the antimicrobial activity of cow urine by peptide and metabolite profiling.Animal bioscience · 2023Article
- Phloretin, as a Potent Anticancer Compound: From Chemistry to Cellular Interactions.Molecules (Basel, Switzerland) · 2022Review
- Ten-Second Electrophysiology: Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis.Scientific reports · 2019Article
- Synthesis, crystal structure, and biological evaluation of a series of phloretin derivatives.Molecules (Basel, Switzerland) · 2014Article
- Measurement of dipole potential in bilayer lipid membranes by dielectric spectroscopy.The Journal of membrane biology · 2014Article
- Depletion of cells and abundant proteins from biological samples by enhanced dielectrophoresis.Sensors and actuators. B, Chemical · 2014Article
- Synthesis, antioxidant and antimicrobial activity of a new phloridzin derivative for dermo-cosmetic applications.Molecules (Basel, Switzerland) · 2012Article
- Atomistic simulation of lipid and DiI dynamics in membrane bilayers under tension.Physical chemistry chemical physics : PCCP · 2011Article
- A combined patch-clamp and electrorotation study of the voltage- and frequency-dependent membrane capacitance caused by structurally dissimilar lipophilic anions.The Journal of membrane biology · 2008Article
- Swelling-activated pathways in human T-lymphocytes studied by cell volumetry and electrorotation.Biophysical journal · 2006Article
- Surviving high-intensity field pulses: strategies for improving robustness and performance of electrotransfection and electrofusion.The Journal of membrane biology · 2005Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The adsorption of the hydrophobic anion [W(CO)(5)CN](-) to human lymphoid Jurkat cells gave rise to an additional anti-field peak in the rotational spectra of single cells, indicating that the cell membrane displayed a strong dielectric dispersion in the kilohertz to megahertz frequency range. The surface concentration of the adsorbed anion and its translocation rate constant between the two membrane boundaries could be evaluated from the rotation spectra of cells by applying the previously proposed mobile charge model. Similar single-cell electrorotation experiments were performed to examine the effect of phloretin, a dipolar molecule known to influence the dipole potential of membranes, on the transport of [W(CO)(5)CN](-) across the plasma membrane of mammalian cells. The adsorption of [W(CO)(5)CN](-) was significantly reduced by phloretin, which is in reasonable agreement with the known phloretin-induced effects on artificial and biological membranes. The IC(50) for the effect of phloretin on the transport parameters of the lipophilic ion was approximately 10 microM. The results of this study are consistent with the assumption that the binding of phloretin reduces the intrinsic dipole potential of the plasma membrane. The experimental approach developed here allows the quantification of intrinsic dipole potential changes within the plasma membrane of living cells.
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